Optical profile: A key determinant of antibacterial efficacy of photodynamic therapy in dentistry
Journal
Photodiagnosis and Photodynamic Therapy
Journal Volume
35
Date Issued
2021
Author(s)
Abstract
Background: Periodontal disease effects 20–50% of the population worldwide, posing a global health challenge. It has been reported to be more prevalent among adults. Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) is an important organism associated with localized juvenile periodontitis. Photodynamic therapy (PDT) has been widely utilized for the treatment of periodontal disease; however, the effect of laser (light) profile on the antibacterial efficacy of PDT remains to be established. The quantitative measurement of laser profile is required to confirm the in vitro efficacy of PDT. Aim: In the present study, a low cost PDT system comprising of six copper tube waveguides (CTW) was developed to provide more uniform irradiation of the culture plate. Methods: The antibacterial effect of PDT, in combination with 200 μg/mL methylene blue (MB) as photosensitizer and 60 sec of irradiation, was studied on A. actinomycetemcomitans and Streptococcus mutans (S. mutans). In the present case, 660 nm laser guided with unpolished CTW, polished CTW, and optical fiber waveguide (OFW) provided radiant exposure of 0.86, 1.38, and 1.36 J/cm2, respectively, for a 24?well culture plate. Results: The designed PDT system provided antimicrobial efficacy of 98% and 91% for A. actinomycetemcomitans and S. mutans, respectively, which was significantly higher as compared to OFW guided PDT. Conclusion: The results of the study highlighted the importance of laser profile as a key parameter that determines the survival rate of bacteria at the edge of the culture plate. Thus, the dose of PDT at the margin of optical profile is important for antibacterial activity for in vitro evaluation. ? 2021 Elsevier B.V.
Subjects
Antimicrobial efficacy
Methylene blue
Optical profile
Periodontal disease
Photodynamic therapy
antiinfective agent
methylene blue
photosensitizing agent
case report
dentistry
human
photochemotherapy
procedures
Anti-Bacterial Agents
Anti-Infective Agents
Dentistry
Humans
Methylene Blue
Photochemotherapy
Photosensitizing Agents
SDGs
Type
journal article