https://scholars.lib.ntu.edu.tw/handle/123456789/598962
標題: | Optical profile: A key determinant of antibacterial efficacy of photodynamic therapy in dentistry | 作者: | Su C.T Chen C.J Chen C.M Chen C.C Ma S.H Wu J.H. CHUNG-MING CHEN |
關鍵字: | Antimicrobial efficacy;Methylene blue;Optical profile;Periodontal disease;Photodynamic therapy;antiinfective agent;methylene blue;photosensitizing agent;case report;dentistry;human;photochemotherapy;procedures;Anti-Bacterial Agents;Anti-Infective Agents;Dentistry;Humans;Methylene Blue;Photochemotherapy;Photosensitizing Agents | 公開日期: | 2021 | 卷: | 35 | 來源出版物: | Photodiagnosis and Photodynamic Therapy | 摘要: | Background: Periodontal disease effects 20–50% of the population worldwide, posing a global health challenge. It has been reported to be more prevalent among adults. Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) is an important organism associated with localized juvenile periodontitis. Photodynamic therapy (PDT) has been widely utilized for the treatment of periodontal disease; however, the effect of laser (light) profile on the antibacterial efficacy of PDT remains to be established. The quantitative measurement of laser profile is required to confirm the in vitro efficacy of PDT. Aim: In the present study, a low cost PDT system comprising of six copper tube waveguides (CTW) was developed to provide more uniform irradiation of the culture plate. Methods: The antibacterial effect of PDT, in combination with 200 μg/mL methylene blue (MB) as photosensitizer and 60 sec of irradiation, was studied on A. actinomycetemcomitans and Streptococcus mutans (S. mutans). In the present case, 660 nm laser guided with unpolished CTW, polished CTW, and optical fiber waveguide (OFW) provided radiant exposure of 0.86, 1.38, and 1.36 J/cm2, respectively, for a 24?well culture plate. Results: The designed PDT system provided antimicrobial efficacy of 98% and 91% for A. actinomycetemcomitans and S. mutans, respectively, which was significantly higher as compared to OFW guided PDT. Conclusion: The results of the study highlighted the importance of laser profile as a key parameter that determines the survival rate of bacteria at the edge of the culture plate. Thus, the dose of PDT at the margin of optical profile is important for antibacterial activity for in vitro evaluation. ? 2021 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111481583&doi=10.1016%2fj.pdpdt.2021.102461&partnerID=40&md5=16138d44d9637a398b9e32c1ce2b748e https://scholars.lib.ntu.edu.tw/handle/123456789/598962 |
ISSN: | 15721000 | DOI: | 10.1016/j.pdpdt.2021.102461 |
顯示於: | 醫學工程學研究所 |
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