Microscale culture and enrichment of cancer stem cells for drug development
Journal
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Pages
1642-1644
Date Issued
2012
Author(s)
Abstract
Screen for drugs that specifically kill epithelial cancer stem cells (CSCs) has not yet been realized due to the rarity of these cells within tumor cell population and their instability in culture [1]. Here we present a microfluidic chip that can facilitate culture and enrichment of cells with CSC-like properties in vitro. Furthermore, we show that human epithelial cancer cells can grow to a tumor spheroid, acquire properties of mesenchymal cells (an increased ability of migration), and have a gain of CSC properties (an increased drug resistance). In addition, this chip requires no external pumps and tubings yet being pipette-friendly in usage.
Subjects
Cancer stem cell; Drug resistance; EMT; Microfluidic; Pipette-friendly; Spheroid
SDGs
Other Subjects
Cell culture; Cell proliferation; Microfluidics; Stem cells; Cancer stem cells; Drug resistance; EMT; Pipette-friendly; Spheroid; Diseases
Publisher
Chemical and Biological Microsystems Society
Type
conference paper