https://scholars.lib.ntu.edu.tw/handle/123456789/504755
標題: | Microscale culture and enrichment of cancer stem cells for drug development | 作者: | Kuo C.-T. Chiang C.-L. Wu C.-T. Huang G.-S. RUBY YUN-JU HUANG Lee H. Wo A.M. |
關鍵字: | Cancer stem cell; Drug resistance; EMT; Microfluidic; Pipette-friendly; Spheroid | 公開日期: | 2012 | 出版社: | Chemical and Biological Microsystems Society | 起(迄)頁: | 1642-1644 | 來源出版物: | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | 摘要: | Screen for drugs that specifically kill epithelial cancer stem cells (CSCs) has not yet been realized due to the rarity of these cells within tumor cell population and their instability in culture [1]. Here we present a microfluidic chip that can facilitate culture and enrichment of cells with CSC-like properties in vitro. Furthermore, we show that human epithelial cancer cells can grow to a tumor spheroid, acquire properties of mesenchymal cells (an increased ability of migration), and have a gain of CSC properties (an increased drug resistance). In addition, this chip requires no external pumps and tubings yet being pipette-friendly in usage. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901769517&partnerID=40&md5=fa8f33f66ed3ab7bb9ae450c25b0a0f8 https://scholars.lib.ntu.edu.tw/handle/123456789/504755 |
ISSN: | #VALUE! | SDG/關鍵字: | Cell culture; Cell proliferation; Microfluidics; Stem cells; Cancer stem cells; Drug resistance; EMT; Pipette-friendly; Spheroid; Diseases |
顯示於: | 醫學系 |
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