Depth-dependent in vivo human skin backscattering spectra extraction from full-field optical coherence tomography
Journal
Journal of Biophotonics
Journal Volume
15
Journal Issue
1
Date Issued
2022
Author(s)
Abstract
With homemade active crystalline fibers, we generated bright and broadband light sources for full-field optical coherence tomography, offering deep penetration into skin tissues with cellular resolution at a high frame rate. Extraction of backscattered spectra from the tissue has potential applications in biomedicine. The hysteresis nonlinearity of the piezoelectric transducer actuating the Mirau interferometer has been greatly reduced by a feedforward compensation approach. The linearized hysteresis response enables us to extract depth-dependent spectra accurately. To validate, the complex dispersion of a fused silica plate was characterized with 2% error. Further validation on an in vitro setting, the backscattered spectra from indocyanine green pigment and nonpigmented microspheres were obtained and verified. For in vivo skin measurement, the backscattered spectra show depth-dependent spectral shift and bandwidth variation due to the complex skin anatomy and pigment absorption. Such a high-speed spectra acquisition of in vivo deep tissue backscattering could lead to disease diagnosis in clinical settings. ? 2021 Wiley-VCH GmbH.
Subjects
Absorption spectroscopy
Backscattering
Diagnosis
Extraction
Fused silica
Hysteresis
Light sources
Optical data processing
Tissue
Back-scattered
Backscattering spectra
Depth dependents
Full-field optical coherence tomographies
Images processing
In-vivo
In-vivo human skin
Spectra's
Tomographic image processing
Tomographic images
Optical tomography
diagnostic imaging
human
optical coherence tomography
skin
Humans
Skin
Tomography, Optical Coherence
SDGs
Type
journal article