6-shogaol induces autophagic cell death then triggered apoptosis in colorectal adenocarcinoma HT-29 cells
Journal
Biomedicine and Pharmacotherapy
Journal Volume
93
Pages
208-217
Date Issued
2017
Author(s)
Li T.-Y.
Abstract
6-shogaol is a phytochemical of dietary ginger, we found that 6-shogaol could induced both autophagic and apoptotic death in human colon adenocarcinoma (HT-29) cells. Results of this study showed that 6-shogal induced cell cycle arrest, autophagy, and apoptosis in HT-29 cells in a time sequence. After 6?h, 6-shogal induced apparent G2/M arrest, then the HT-29 cells formed numerous autophagosomes in each phase of the cell cycle. After 18?h, increases in acidic vesicles and LAMP-1 (Lysosome-associated membrane proteins 1) showed that 6-shogaol had caused autophagic cell death. After 24?h, cell shrinkage and Caspase-3/7 activities rising, suggesting that apoptotic cell death had increased. And after 48?h, the result of TUNEL assay indicated the highest occurrence of apoptosis upon 6-shogaol treatment. It appeared that apoptosis is triggered by autophagy in 6-shogaol treated HT-29 cells, the damage of autophagic cell death initiated apoptosis program. ? 2017 Elsevier Masson SAS
Subjects
6-shogaol
Apoptosis
Autophagy
Colorectal cancer
HT 29
SDGs
Other Subjects
caspase 3; caspase 7; shogaol; caspase 3; caspase 7; catechol derivative; reactive oxygen metabolite; shogaol; apoptosis; Article; autolysosome; autophagosome; autophagy; cell culture; cell cycle arrest; cell cycle assay; cell death; cell viability; colorectal carcinoma; controlled study; DNA damage; enzyme activity; G2 phase cell cycle checkpoint; HT-29 cell line; human; human cell; immunocytochemistry; lysosome membrane; M phase cell cycle checkpoint; priority journal; TUNEL assay; adenocarcinoma; apoptosis; cell cycle checkpoint; cell death; chemistry; colorectal tumor; drug effects; ginger; metabolism; signal transduction; tumor cell line; Adenocarcinoma; Apoptosis; Autophagy; Caspase 3; Caspase 7; Catechols; Cell Cycle Checkpoints; Cell Death; Cell Line, Tumor; Colorectal Neoplasms; G2 Phase Cell Cycle Checkpoints; Ginger; HT29 Cells; Humans; Reactive Oxygen Species; Signal Transduction
Type
journal article