|Title:||6-shogaol induces autophagic cell death then triggered apoptosis in colorectal adenocarcinoma HT-29 cells||Authors:||Li T.-Y.
|Keywords:||6-shogaol;Apoptosis;Autophagy;Colorectal cancer;HT 29||Issue Date:||2017||Source:||Biomedicine and Pharmacotherapy||Journal Volume:||93||Start page/Pages:||208-217||Abstract:||
6-shogaol is a phytochemical of dietary ginger, we found that 6-shogaol could induced both autophagic and apoptotic death in human colon adenocarcinoma (HT-29) cells. Results of this study showed that 6-shogal induced cell cycle arrest, autophagy, and apoptosis in HT-29 cells in a time sequence. After 6?h, 6-shogal induced apparent G2/M arrest, then the HT-29 cells formed numerous autophagosomes in each phase of the cell cycle. After 18?h, increases in acidic vesicles and LAMP-1 (Lysosome-associated membrane proteins 1) showed that 6-shogaol had caused autophagic cell death. After 24?h, cell shrinkage and Caspase-3/7 activities rising, suggesting that apoptotic cell death had increased. And after 48?h, the result of TUNEL assay indicated the highest occurrence of apoptosis upon 6-shogaol treatment. It appeared that apoptosis is triggered by autophagy in 6-shogaol treated HT-29 cells, the damage of autophagic cell death initiated apoptosis program. ? 2017 Elsevier Masson SAS
|Appears in Collections:||食品科技研究所|
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