https://scholars.lib.ntu.edu.tw/handle/123456789/413274
標題: | 6-shogaol induces autophagic cell death then triggered apoptosis in colorectal adenocarcinoma HT-29 cells | 作者: | Li T.-Y. Chiang B.-H. |
關鍵字: | 6-shogaol;Apoptosis;Autophagy;Colorectal cancer;HT 29 | 公開日期: | 2017 | 卷: | 93 | 起(迄)頁: | 208-217 | 來源出版物: | Biomedicine and Pharmacotherapy | 摘要: | 6-shogaol is a phytochemical of dietary ginger, we found that 6-shogaol could induced both autophagic and apoptotic death in human colon adenocarcinoma (HT-29) cells. Results of this study showed that 6-shogal induced cell cycle arrest, autophagy, and apoptosis in HT-29 cells in a time sequence. After 6?h, 6-shogal induced apparent G2/M arrest, then the HT-29 cells formed numerous autophagosomes in each phase of the cell cycle. After 18?h, increases in acidic vesicles and LAMP-1 (Lysosome-associated membrane proteins 1) showed that 6-shogaol had caused autophagic cell death. After 24?h, cell shrinkage and Caspase-3/7 activities rising, suggesting that apoptotic cell death had increased. And after 48?h, the result of TUNEL assay indicated the highest occurrence of apoptosis upon 6-shogaol treatment. It appeared that apoptosis is triggered by autophagy in 6-shogaol treated HT-29 cells, the damage of autophagic cell death initiated apoptosis program. ? 2017 Elsevier Masson SAS |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413274 | ISSN: | 07533322 | DOI: | 10.1016/j.biopha.2017.06.038 | SDG/關鍵字: | caspase 3; caspase 7; shogaol; caspase 3; caspase 7; catechol derivative; reactive oxygen metabolite; shogaol; apoptosis; Article; autolysosome; autophagosome; autophagy; cell culture; cell cycle arrest; cell cycle assay; cell death; cell viability; colorectal carcinoma; controlled study; DNA damage; enzyme activity; G2 phase cell cycle checkpoint; HT-29 cell line; human; human cell; immunocytochemistry; lysosome membrane; M phase cell cycle checkpoint; priority journal; TUNEL assay; adenocarcinoma; apoptosis; cell cycle checkpoint; cell death; chemistry; colorectal tumor; drug effects; ginger; metabolism; signal transduction; tumor cell line; Adenocarcinoma; Apoptosis; Autophagy; Caspase 3; Caspase 7; Catechols; Cell Cycle Checkpoints; Cell Death; Cell Line, Tumor; Colorectal Neoplasms; G2 Phase Cell Cycle Checkpoints; Ginger; HT29 Cells; Humans; Reactive Oxygen Species; Signal Transduction |
顯示於: | 食品科技研究所 |
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