Macroenvironmental interactions as driving indicators for detecting tetracycline resistance spread among A. hydrophila exposure to environmentally relevant oxytetracycline levels
Journal
Ecological Indicators
Journal Volume
146
Date Issued
2023-02-01
Author(s)
Abstract
There have been efforts dedicating to investigating the effect of high levels of oxytetracycline (OTC) on tetracycline (TC) resistance among zoonotic pathogen Aeromonas hydrophila (AH), an antibiotic-resistant bacterial indicator. However, the dynamic behavior of TC-resistant AH in response to environmentally relevant OTC concentrations at a population-level has not been fully understood. Here we developed a bacterial population dynamic model to quantify TC-resistant AH posed by OTC-dependent resistance selection pressure. The key environmental factors known as water temperature, water activity, and pH were incorporated into model to produce pattern-oriented simulation outcomes. We estimated resistance acquisition number (R0) and showed that R0 was >1 at water temperature <26 °C, indicating coexistence of resistant- and susceptible-AH. Sensitivity tests revealed that cell density-dependent conjugation rate indicated crucial for influencing R0 estimation. Our results also indicated that maximum fraction of TC-resistant AH was mostly affected by temperature/activity in water and increased with increasing of OTC concentrations. We estimated OTC concentrations causing 50 % maximum fold-change of TC-resistant AH fraction ranging from 7 to 19 µg/L. Our findings suggest that control of TC resistance in AH requires particularly attention to water with temperature 26 °C lower, water activity 0.95 higher with pH ∼ 5–8. Our mechanistic framework provides a useful tool-kit to improve our understanding of the critical role of OTC stress-induced macroenvironmental interactions in a TC resistance − AH system and highlights the potential for antimicrobial management to promote resilience in aquatic ecosystems.
Subjects
Aeromonas hydrophila | Aquatic ecosystems | Macroenvironmental interactions | One Health | Oxytetracycline | Tetracycline resistance
Publisher
ELSEVIER
Type
journal article