https://scholars.lib.ntu.edu.tw/handle/123456789/629827
標題: | Macroenvironmental interactions as driving indicators for detecting tetracycline resistance spread among A. hydrophila exposure to environmentally relevant oxytetracycline levels | 作者: | Lu, Tien Hsuan Chen, Chi Yun Wang, Wei Min CHUNG-MIN LIAO |
關鍵字: | Aeromonas hydrophila | Aquatic ecosystems | Macroenvironmental interactions | One Health | Oxytetracycline | Tetracycline resistance | 公開日期: | 1-二月-2023 | 出版社: | ELSEVIER | 卷: | 146 | 來源出版物: | Ecological Indicators | 摘要: | There have been efforts dedicating to investigating the effect of high levels of oxytetracycline (OTC) on tetracycline (TC) resistance among zoonotic pathogen Aeromonas hydrophila (AH), an antibiotic-resistant bacterial indicator. However, the dynamic behavior of TC-resistant AH in response to environmentally relevant OTC concentrations at a population-level has not been fully understood. Here we developed a bacterial population dynamic model to quantify TC-resistant AH posed by OTC-dependent resistance selection pressure. The key environmental factors known as water temperature, water activity, and pH were incorporated into model to produce pattern-oriented simulation outcomes. We estimated resistance acquisition number (R0) and showed that R0 was >1 at water temperature <26 °C, indicating coexistence of resistant- and susceptible-AH. Sensitivity tests revealed that cell density-dependent conjugation rate indicated crucial for influencing R0 estimation. Our results also indicated that maximum fraction of TC-resistant AH was mostly affected by temperature/activity in water and increased with increasing of OTC concentrations. We estimated OTC concentrations causing 50 % maximum fold-change of TC-resistant AH fraction ranging from 7 to 19 µg/L. Our findings suggest that control of TC resistance in AH requires particularly attention to water with temperature 26 °C lower, water activity 0.95 higher with pH ∼ 5–8. Our mechanistic framework provides a useful tool-kit to improve our understanding of the critical role of OTC stress-induced macroenvironmental interactions in a TC resistance − AH system and highlights the potential for antimicrobial management to promote resilience in aquatic ecosystems. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/629827 | ISSN: | 1470160X | DOI: | 10.1016/j.ecolind.2023.109873 |
顯示於: | 生物環境系統工程學系 |
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