Monitoring and control of inertial cavitation activity for enhancing ultrasound transfection: The SonInCaRe project
Journal
IRBM
Journal Volume
35
Journal Issue
2
Pages
94-99
Date Issued
2014
Author(s)
Inserra C.
Labelle P.
Der Loughian C.
Lee J.-L.
Fouqueray M.
Ngo J.
Poizat A.
Desjouy C.
Munteanu B.
Lo C.-W.
Vanbelle C.
Rieu J.-P.
Béra J.-C.
Abstract
Sonoporation process, at the origin of ultrasound cell transfection, is ruled by the interaction between cells and cavitating bubbles. Due to the stochastic behavior of acoustic cavitation, there exists a need in ensuring a stable state of cavitation within a medium during cell transfection to enhance transfection efficiency and control mortality. The goal of the SonInCaRe project is thus to define a controlled-cavitation device in order to monitor, control and stabilize inertial cavitation activity during cell sonication in real-time. This device, based on a feedback loop acting in real-time, allows ensuring fixed cavitation conditions during a pulsed sonication. Its application to suspended and adherent cells transfection shows better reproducibility compared to the fixed acoustic intensity sonication. The regulation device thus provides a better control of cavitation activity and its bioeffects which are of crucial importance for clinical applications of ultrasound-mediated gene transfection. ? 2014 Elsevier Masson SAS.
SDGs
Other Subjects
adherent cell; article; feedback system; genetic transfection; human; human cell; lipid bilayer; oscillation; reproducibility; sound intensity; ultrasound; ultrasound cell transfection
Publisher
Elsevier Masson SAS
Type
journal article