https://scholars.lib.ntu.edu.tw/handle/123456789/481421
標題: | Monitoring and control of inertial cavitation activity for enhancing ultrasound transfection: The SonInCaRe project | 作者: | Inserra C. Labelle P. Der Loughian C. Lee J.-L. Fouqueray M. Ngo J. Poizat A. Desjouy C. Munteanu B. Lo C.-W. Vanbelle C. Rieu J.-P. WEN-SHIANG CHEN Béra J.-C. |
公開日期: | 2014 | 出版社: | Elsevier Masson SAS | 卷: | 35 | 期: | 2 | 起(迄)頁: | 94-99 | 來源出版物: | IRBM | 摘要: | Sonoporation process, at the origin of ultrasound cell transfection, is ruled by the interaction between cells and cavitating bubbles. Due to the stochastic behavior of acoustic cavitation, there exists a need in ensuring a stable state of cavitation within a medium during cell transfection to enhance transfection efficiency and control mortality. The goal of the SonInCaRe project is thus to define a controlled-cavitation device in order to monitor, control and stabilize inertial cavitation activity during cell sonication in real-time. This device, based on a feedback loop acting in real-time, allows ensuring fixed cavitation conditions during a pulsed sonication. Its application to suspended and adherent cells transfection shows better reproducibility compared to the fixed acoustic intensity sonication. The regulation device thus provides a better control of cavitation activity and its bioeffects which are of crucial importance for clinical applications of ultrasound-mediated gene transfection. ? 2014 Elsevier Masson SAS. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898413632&doi=10.1016%2fj.irbm.2014.02.010&partnerID=40&md5=de22768296a3b3c549bdbce8eafc89c7 https://scholars.lib.ntu.edu.tw/handle/123456789/481421 |
ISSN: | 1959-0318 | DOI: | 10.1016/j.irbm.2014.02.010 | SDG/關鍵字: | adherent cell; article; feedback system; genetic transfection; human; human cell; lipid bilayer; oscillation; reproducibility; sound intensity; ultrasound; ultrasound cell transfection |
顯示於: | 醫學系 |
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