Determining mechanical stimulation responses of primary cilia with an integrated microfluidics platform
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Journal Volume
3
Pages
1513-1516
Date Issued
2018
Author(s)
Abstract
We developed a microfluidic platform to stimulate primary cilia and observe its bending mechanism in order to study the mechanosensation of primary cilium. The microfluidic device is resealable for cell culture and immunostaining process. Besides, flow conditions, such as flow rates, flow directions and in a single channel can be well-controlled to stimulate a large number of primary cilia. Moreover, the microfluidic platform can directly change different fluids using a three-way valves, which enables the direct primary cilia fixation without stopping the fluid flow. To proof-of-concept, we apply various flow rates flow to stimulate the primary cilium and observe its bending phenomenon. Furthermore, we immunostained over 100 primary cilia in each flow rate to perform a large number bending behavior analysis. In summary, our system is capable of bending multiple primary cilia at the same time and detachable after flow experiment for further analysis. This microfluidic platform presents the ability to stimulate primary cilia and can be integrated with other technique like superresolution microscopy and protein mass spectrometry to study how fluid flow affect both the external structure and the inner biological responses. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Subjects
Cell culture; Fixed platforms; Flow of fluids; Mass spectrometry; Biological response; Integrated microfluidics; Mechanical stimulation; Mechanosensation; Micro-fluidic devices; Microfluidic platforms; Primary cilium; Super-resolution microscopy; Microfluidics
Type
conference paper