Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines
Journal
Cancer Letters
Journal Volume
315
Journal Issue
1
Pages
1-11
Date Issued
2012
Author(s)
Abstract
In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins. ? 2011 Elsevier Ireland Ltd.
SDGs
Other Subjects
cyclin B1; cyclin dependent kinase 1; protein bcl 2; protein mcl 1; protopine; antineoplastic activity; apoptosis; article; cancer cell culture; cell cycle arrest; cell cycle G2 phase; cell cycle M phase; cell cycle progression; cell cycle regulation; cell proliferation; concentration response; controlled study; down regulation; drug cytotoxicity; drug structure; drug synthesis; enzyme activation; enzyme activity; human; human cell; in vitro study; intracellular signaling; microtubule assembly; mitochondrion; priority journal; prostate cancer; protein expression; protein phosphorylation; Apoptosis; Benzophenanthridines; Berberine Alkaloids; Cell Growth Processes; Cell Line, Tumor; Humans; Male; Mitosis; Neoplasms, Hormone-Dependent; Prostatic Neoplasms; Signal Transduction; Transfection; Tubulin Modulators
Type
journal article