Effect of Epstein-Barr virus infection on global gene expression in nasopharyngeal carcinoma
Journal
Functional and Integrative Genomics
Journal Volume
7
Journal Issue
1
Pages
79-93
Date Issued
2007
Author(s)
Lee Y.-C.G.
Hwang Y.-C.
Chen K.-C.
Lin Y.-S.
Huang D.-Y.
Huang T.-W.
Kao C.-Y.
Wu H.-C.
Huang C.-Y.F.
Abstract
It was proposed that Epstein-Barr virus (EBV) is closely associated with nasopharyngeal carcinoma (NPC); however, the molecular mechanisms involved in the effect of EBV on NPC host genes have not yet been well defined. For this study, two sets of microarray experiments, NPC (EBV-free) vs normal epithelial cells and EBV+ vs EBV- NPC arrays, were analyzed and the datasets were cross-compared to identify any correlation between gene clusters involved in EBV targeting and the NPC host gene expression profiles. Statistical analysis revealed that EBV seems to have a preference for targeting more genes from the differentially expressed group in NPC cells than those from the ubiquitously expressed group. Furthermore, this trend is also reflected in log ratios where the EBV target genes of the differentially expressed group origin showed greater log ratios than genes with an origin from the ubiquitously expressed NPC group. Taken together, the genome-wide comparative scanning of EBV and NPC transcriptomes has successfully demonstrated that EBV infection has an intensifying effect on the signals involved in NPC gene expression both in breadth (the majority of the genes) and in depth (greater log ratios). ? Springer-Verlag 2006.
Subjects
Epstein-Barr virus (EBV); Nasopharyngeal carcinoma (NPC); Quantitative real-time reverse transcriptase polymerase chain reaction (Q-RT-PCR); Transcriptomes
SDGs
Other Subjects
actin; binding protein; caspase 3; cell antigen; death associated protein kinase; midkine; Myc protein; myosin light chain; protein kinase C; somatomedin binding protein; syndecan; syntrophin; transcriptome; transforming growth factor beta1; animal cell; animal experiment; animal model; animal tissue; article; controlled study; correlation analysis; disease association; epithelium cell; Epstein Barr virus; gene cluster; gene expression; gene expression profiling; gene targeting; host; human; human cell; microarray analysis; molecular mechanics; mouse; nasopharynx carcinoma; nonhuman; nucleotide sequence; priority journal; reverse transcription polymerase chain reaction; signal transduction; statistical analysis; virus infection; Carcinoma; Cell Line; Cells, Cultured; Epstein-Barr Virus Infections; Gene Expression Regulation, Neoplastic; Herpesvirus 4, Human; Humans; Nasopharyngeal Neoplasms; Human herpesvirus 4
Type
journal article