Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
10251
Pages
1025109
ISBN
9781510610033
Date Issued
2017-01-01
Author(s)
Abstract
Three-dimensional (3D) refractive-index (RI) microscopy is an emerging technique suitable for live-cell imaging due to its label-free and fast 3D imaging capabilities. We have developed a common-path system to acquire 3D RI microscopic images of cells with excellent speed and stability. After obtaining 3D RI distributions of individual leukocytes, we used a 3D finite-difference time-domain tool to study light scattering properties. Backscattering spectra of lymphocytes, monocytes and neutrophils are different from each other. Backscattering spectra of lymphocytes matched well with those of homogeneous spheres as predicted by Mie theory while backscattering spectra of neutrophils are significantly more intense than those of the other two types. This suggests the possibility of classifying the three types of leukocytes based on backscattering.
Subjects
Backscattering spectra | Finite-difference time-domain method | Leukocytes | Tomographic phase microscopy
Type
conference paper