https://scholars.lib.ntu.edu.tw/handle/123456789/629023
標題: | Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps | 作者: | KUNG-BIN SUNG Lin, Yang Hsien Lin, Fong Jheng Hsieh, Chao Mao SHANG-JU WU |
關鍵字: | Backscattering spectra | Finite-difference time-domain method | Leukocytes | Tomographic phase microscopy | 公開日期: | 1-一月-2017 | 卷: | 10251 | 起(迄)頁: | 1025109 | 來源出版物: | Proceedings of SPIE - The International Society for Optical Engineering | 摘要: | Three-dimensional (3D) refractive-index (RI) microscopy is an emerging technique suitable for live-cell imaging due to its label-free and fast 3D imaging capabilities. We have developed a common-path system to acquire 3D RI microscopic images of cells with excellent speed and stability. After obtaining 3D RI distributions of individual leukocytes, we used a 3D finite-difference time-domain tool to study light scattering properties. Backscattering spectra of lymphocytes, monocytes and neutrophils are different from each other. Backscattering spectra of lymphocytes matched well with those of homogeneous spheres as predicted by Mie theory while backscattering spectra of neutrophils are significantly more intense than those of the other two types. This suggests the possibility of classifying the three types of leukocytes based on backscattering. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/629023 | ISBN: | 9781510610033 | ISSN: | 0277786X | DOI: | 10.1117/12.2270427 |
顯示於: | 醫學系 |
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