Treatment of osteoarthritis with collagen-based scaffold: A porcine animal model with xenograft mesenchymal stem cells
Journal
Histology and Histopathology
Journal Volume
33
Journal Issue
12
Pages
1271-1286
Date Issued
2019
Author(s)
Huang, S.-W.
Fang, C.-H.
Hsu, L.-T.
Chen, C.-Y.
Shen, H.-H.
Abstract
Summary. Objective. With the goal to explore a new approach to treat the early degenerative lesions of hyaline cartilage, we implanted in a porcine OA model a collagen-based scaffold containing chondroprogenitor cells derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Experimental design. Porcine knee joints were subjected to anterior cruciate ligament (ACL) transection to surgically induce OA. After 4 months, the time necessary for the development of cartilage surface damage, animals were treated either wi t h t r ephi nat i on bone pl ug wr apped wi t h t he chondroprogeni c hBM-MSCs-embedded col l agen scaffold or microfractures alone. Histological and histomorphometric evaluations were performed at 5 months after surgery. Results. All animals subjected to ACL t r ansect i on showed ost eoar t hr i t i c changes including mild lateral femoral condyle or moderate medial femoral condyle ulcerations. After 14 days’ chondrogenic induction, hBM-MSCs seeded onto the scaffold showed expression of chondroprogeni tor markers such as SOX9 and COMP. At 5 months after the implantation, significant differences in the quality of the regenerated tissue were found between the hBM-MSCs-embedded scaffold group and the control group. Newly generat ed t i ssue was onl y observed at t he si t e of implantation with the hBM-MSCs-embedded scaffolds. Furthermore, histological examination of the generated tissue revealed evidence of cartilage-like tissue with lacuna formation. In contrast, fibrous layers or fissures were formed on the surface of the control knee joint. Conclusions. This study shows that xenogenic hBM-MSC derived chondroprogenitor scaffolds can generate new cartilage tissue in porcine articular cartilage and have the potential as a useful treatment option for osteoarthritis. ? Histology and Histopathology. All rights reserved.
SDGs
Other Subjects
collagen; animal; articular cartilage; chondrogenesis; disease model; human; mesenchymal stem cell transplantation; osteoarthritis; physiology; pig; procedures; tissue engineering; tissue scaffold; xenograft; Animals; Cartilage, Articular; Chondrogenesis; Collagen; Disease Models, Animal; Heterografts; Humans; Mesenchymal Stem Cell Transplantation; Osteoarthritis; Swine; Tissue Engineering; Tissue Scaffolds
Publisher
Histology and Histopathology
Type
journal article