https://scholars.lib.ntu.edu.tw/handle/123456789/455293
標題: | Treatment of osteoarthritis with collagen-based scaffold: A porcine animal model with xenograft mesenchymal stem cells | 作者: | WO-JAN TSENG Huang, S.-W. Fang, C.-H. Hsu, L.-T. Chen, C.-Y. Shen, H.-H. JENNY ZWEI-CHIENG CHANG JUI-SHENG SUN FENG-HUEI LIN |
公開日期: | 2019 | 出版社: | Histology and Histopathology | 卷: | 33 | 期: | 12 | 起(迄)頁: | 1271-1286 | 來源出版物: | Histology and Histopathology | 摘要: | Summary. Objective. With the goal to explore a new approach to treat the early degenerative lesions of hyaline cartilage, we implanted in a porcine OA model a collagen-based scaffold containing chondroprogenitor cells derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Experimental design. Porcine knee joints were subjected to anterior cruciate ligament (ACL) transection to surgically induce OA. After 4 months, the time necessary for the development of cartilage surface damage, animals were treated either wi t h t r ephi nat i on bone pl ug wr apped wi t h t he chondroprogeni c hBM-MSCs-embedded col l agen scaffold or microfractures alone. Histological and histomorphometric evaluations were performed at 5 months after surgery. Results. All animals subjected to ACL t r ansect i on showed ost eoar t hr i t i c changes including mild lateral femoral condyle or moderate medial femoral condyle ulcerations. After 14 days’ chondrogenic induction, hBM-MSCs seeded onto the scaffold showed expression of chondroprogeni tor markers such as SOX9 and COMP. At 5 months after the implantation, significant differences in the quality of the regenerated tissue were found between the hBM-MSCs-embedded scaffold group and the control group. Newly generat ed t i ssue was onl y observed at t he si t e of implantation with the hBM-MSCs-embedded scaffolds. Furthermore, histological examination of the generated tissue revealed evidence of cartilage-like tissue with lacuna formation. In contrast, fibrous layers or fissures were formed on the surface of the control knee joint. Conclusions. This study shows that xenogenic hBM-MSC derived chondroprogenitor scaffolds can generate new cartilage tissue in porcine articular cartilage and have the potential as a useful treatment option for osteoarthritis. ? Histology and Histopathology. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057553019&doi=10.14670%2fHH-18-013&partnerID=40&md5=d01637e2353cf879ad56b3d125c7a61b https://scholars.lib.ntu.edu.tw/handle/123456789/455293 |
ISSN: | 0213-3911 | DOI: | 10.14670/HH-18-013 | SDG/關鍵字: | collagen; animal; articular cartilage; chondrogenesis; disease model; human; mesenchymal stem cell transplantation; osteoarthritis; physiology; pig; procedures; tissue engineering; tissue scaffold; xenograft; Animals; Cartilage, Articular; Chondrogenesis; Collagen; Disease Models, Animal; Heterografts; Humans; Mesenchymal Stem Cell Transplantation; Osteoarthritis; Swine; Tissue Engineering; Tissue Scaffolds |
顯示於: | 醫學系 |
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