Pterostilbene Attenuates High-Fat Diet and Dextran Sulfate Sodium-Induced Colitis via Suppressing Inflammation and Intestinal Fibrosis in Mice
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
69
Journal Issue
25
Pages
7093-7103
Date Issued
2021
Author(s)
Abstract
The worldwide prevalence of obesity has significantly increased over the past few decades. It is currently believed that obesity is a risk factor for developing inflammatory bowel disease. Pterostilbene (PTS), a naturally occurring stilbene from blueberries, is known to have anticancer, anti-inflammation, antifibrosis, and antiobesity effects. The preventive effect of PTS on the susceptibility of high-fat diet (HFD) to dextran sulfate sodium (DSS)-induced colitis in mice was investigated. Beginning at 5 weeks of age, C57BL/6J mice were fed a normal diet, 50% HFD alone, or containing PTS, and DSS (2.5%, w/v) was given in drinking water at week 9 and week 11. The results demonstrated that PTS significantly attenuated HFD and DSS-induced plasma interleukin-6 accumulation. Moreover, PTS suppressed HFD/DSS-induced formation of aberrant crypt foci and reduced the colon weight-to-length ratio in HFD/DSS-induced colitis mice. Furthermore, PTS inhibited interleukin-1β (IL-1β), the C/EBP homologous protein (CHOP), cyclooxygenase-2, and transforming growth factor beta-1 (TGF-β1)/mothers against decapentaplegic homolog 2 expression and maintained mucin2 (Muc2) and E-cadherin expressions. In addition, post-treatment with PTS also decreased the colon weight-to-length ratio and loss of Muc2. Moreover, the CHOP, IL-1β, matrix metalloproteinase-2, and TGF-β1 expressions were significantly decreased in HFD/DSS-induced colitis mice after post-treatment with PTS. In conclusion, the results of the present study suggest that PTS is of significant interest for the prevention of HFD/DSS-induced colitis in C57BL/6J mice. ?
Subjects
colitis
dextran sulfate sodium (DSS)
high-fat diet (HFD)
intestinal fibrosis
pterostilbene (PTS)
Dextran
Mammals
Nutrition
Pathology
Potable water
Sodium
Sulfur compounds
Dextran sulfate sodium
Dextran sulfate sodium (DSS) induced colitis
E-cadherin expression
Homologous proteins
Inflammatory bowel disease
Matrix metalloproteinase-2
Naturally occurring
Transforming growth factor beta
Diseases
dextran sulfate
gelatinase A
pterostilbene
stilbene derivative
adverse event
animal
C57BL mouse
colon
disease model
fibrosis
genetics
inflammation
lipid diet
mouse
pathology
Animals
Colitis
Colon
Dextran Sulfate
Diet, High-Fat
Disease Models, Animal
Fibrosis
Inflammation
Matrix Metalloproteinase 2
Mice
Mice, Inbred C57BL
Stilbenes
SDGs
Type
journal article