EGFR-targeted micelles containing near-infrared dye for enhanced photothermal therapy in colorectal cancer
Journal
Journal of Controlled Release
Journal Volume
258
Pages
196-207
Date Issued
2017
Author(s)
Abstract
The purpose of this research was to investigate the effectiveness of epidermal growth factor receptor (EGFR) targeted micelles loaded with IR-780 (Cetuximab/IR-780/micelles) for generating tumor targeting, multimodal images, and photothermal therapy (PTT). We initially studied the cellular uptake of these micelles using the HCT-116 and SW-620 cell lines. HCT-116 (high expression of EGFR) and SW-620 (low expression of EGFR) cell lines were used to examine biodistribution and antitumor effects of Cetuximab/IR-780/micelles. Time-lapse near-IR fluorescence (NIRF) images also indicated the highest IR-780 accumulation from Cetuximab/IR-780/micelles in HCT-116 tumors (p < 0.05). HCT-116 tumors in tumor-bearing mice exhibited significantly higher accumulations of Cetuximab/IR-780/111In-micelles than SW-620 tumors in Micro-SPECT/CT imaging and biodistribution studies (p < 0.05). Dual-radioisotope Nano-SPECT/CT imaging of Cetuximab/131I-IR-780/111In-micelles demonstrated simultaneous high accumulation of both IR-780 and micelles in HCT-116 tumors, but not in SW-620 tumors. Regarding antitumor effects, following the Cetuximab/IR-780/micelles with PPT on day 6, all HCT-116 tumor-bearing mice were cured. In contrast, SW-620 tumors relapsed at 13 days after treatment. In summary, we expect that the Cetuximab/IR-780/micelles could enhance the antitumor effects by PTT in EGFR overexpression colorectal cancers through effective drug delivery nanoparticles. ? 2017 Elsevier B.V.
Subjects
Colorectal carcinoma; Dual isotope image; Photosensitizer; Photothermal
SDGs
Other Subjects
Cell culture; Diseases; Infrared devices; Mammals; Micelles; Photosensitizers; Colorectal carcinoma; Drug delivery nanoparticle; Dual isotope image; Epidermal growth factor receptors; Near-infrared dyes; Near-IR fluorescence; Photo-thermal; Photothermal therapy; Tumors; cetuximab; epidermal growth factor receptor; indium 111; iodine 131; ir 780; maleimide; photosensitizing agent; sepharose; unclassified drug; 2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium; cetuximab; epidermal growth factor receptor; immunological antineoplastic agent; indole derivative; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; Article; cellular distribution; colorectal cancer; confocal microscopy; controlled study; CT-SPECT scanner; female; flow cytometry; fluorescence imaging; HCT 116 cell line; immunohistochemistry; isotope labeling; micelle; mouse; near infrared fluorescence imaging; nonhuman; particle size; photothermal therapy; polymerization; priority journal; protein expression; protein targeting; proton nuclear magnetic resonance; radiochemistry; single photon emission computed tomography-computed tomography; animal; autoradiography; colorectal tumor; diagnostic imaging; drug delivery system; human; metabolism; micelle; nude mouse; phototherapy; procedures; single photon emission computed tomography; thermotherapy; tissue distribution; Animals; Antineoplastic Agents, Immunological; Autoradiography; Cetuximab; Colorectal Neoplasms; Drug Delivery Systems; Female; HCT116 Cells; Humans; Hyperthermia, Induced; Indoles; Mice, Nude; Micelles; Phototherapy; Receptor, Epidermal Growth Factor; Tissue Distribution; Tomography, Emission-Computed, Single-Photon
Publisher
Elsevier B.V.
Type
journal article