https://scholars.lib.ntu.edu.tw/handle/123456789/465722
標題: | EGFR-targeted micelles containing near-infrared dye for enhanced photothermal therapy in colorectal cancer | 作者: | Shih Y.-H. Luo T.-Y. Chiang P.-F. Yao C.-J. Lin W.-J. Peng C.-L. MING-JIUM SHIEH |
關鍵字: | Colorectal carcinoma; Dual isotope image; Photosensitizer; Photothermal | 公開日期: | 2017 | 出版社: | Elsevier B.V. | 卷: | 258 | 起(迄)頁: | 196-207 | 來源出版物: | Journal of Controlled Release | 摘要: | The purpose of this research was to investigate the effectiveness of epidermal growth factor receptor (EGFR) targeted micelles loaded with IR-780 (Cetuximab/IR-780/micelles) for generating tumor targeting, multimodal images, and photothermal therapy (PTT). We initially studied the cellular uptake of these micelles using the HCT-116 and SW-620 cell lines. HCT-116 (high expression of EGFR) and SW-620 (low expression of EGFR) cell lines were used to examine biodistribution and antitumor effects of Cetuximab/IR-780/micelles. Time-lapse near-IR fluorescence (NIRF) images also indicated the highest IR-780 accumulation from Cetuximab/IR-780/micelles in HCT-116 tumors (p < 0.05). HCT-116 tumors in tumor-bearing mice exhibited significantly higher accumulations of Cetuximab/IR-780/111In-micelles than SW-620 tumors in Micro-SPECT/CT imaging and biodistribution studies (p < 0.05). Dual-radioisotope Nano-SPECT/CT imaging of Cetuximab/131I-IR-780/111In-micelles demonstrated simultaneous high accumulation of both IR-780 and micelles in HCT-116 tumors, but not in SW-620 tumors. Regarding antitumor effects, following the Cetuximab/IR-780/micelles with PPT on day 6, all HCT-116 tumor-bearing mice were cured. In contrast, SW-620 tumors relapsed at 13 days after treatment. In summary, we expect that the Cetuximab/IR-780/micelles could enhance the antitumor effects by PTT in EGFR overexpression colorectal cancers through effective drug delivery nanoparticles. ? 2017 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020191827&doi=10.1016%2fj.jconrel.2017.04.031&partnerID=40&md5=a4335db9181d91bd58157e0643f4ae89 https://scholars.lib.ntu.edu.tw/handle/123456789/465722 |
ISSN: | 0168-3659 | DOI: | 10.1016/j.jconrel.2017.04.031 | SDG/關鍵字: | Cell culture; Diseases; Infrared devices; Mammals; Micelles; Photosensitizers; Colorectal carcinoma; Drug delivery nanoparticle; Dual isotope image; Epidermal growth factor receptors; Near-infrared dyes; Near-IR fluorescence; Photo-thermal; Photothermal therapy; Tumors; cetuximab; epidermal growth factor receptor; indium 111; iodine 131; ir 780; maleimide; photosensitizing agent; sepharose; unclassified drug; 2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium; cetuximab; epidermal growth factor receptor; immunological antineoplastic agent; indole derivative; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; Article; cellular distribution; colorectal cancer; confocal microscopy; controlled study; CT-SPECT scanner; female; flow cytometry; fluorescence imaging; HCT 116 cell line; immunohistochemistry; isotope labeling; micelle; mouse; near infrared fluorescence imaging; nonhuman; particle size; photothermal therapy; polymerization; priority journal; protein expression; protein targeting; proton nuclear magnetic resonance; radiochemistry; single photon emission computed tomography-computed tomography; animal; autoradiography; colorectal tumor; diagnostic imaging; drug delivery system; human; metabolism; micelle; nude mouse; phototherapy; procedures; single photon emission computed tomography; thermotherapy; tissue distribution; Animals; Antineoplastic Agents, Immunological; Autoradiography; Cetuximab; Colorectal Neoplasms; Drug Delivery Systems; Female; HCT116 Cells; Humans; Hyperthermia, Induced; Indoles; Mice, Nude; Micelles; Phototherapy; Receptor, Epidermal Growth Factor; Tissue Distribution; Tomography, Emission-Computed, Single-Photon |
顯示於: | 醫學工程學研究所 |
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