Protocol for renal ischemia-reperfusion injury by flank incisions in mice
Journal
STAR protocols
Journal Volume
3
Journal Issue
4
Date Issued
2022-10-06
Author(s)
Abstract
Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014).
Subjects
Health Sciences; Metabolism; Model Organisms; Molecular Biology
SDGs
Type
journal article