https://scholars.lib.ntu.edu.tw/handle/123456789/623997
標題: | Protocol for renal ischemia-reperfusion injury by flank incisions in mice | 作者: | Cheng, Yung-Ting Tu, Ya-Chun YU-HSIANG CHOU CHUN-FU LAI |
關鍵字: | Health Sciences; Metabolism; Model Organisms; Molecular Biology | 公開日期: | 6-十月-2022 | 卷: | 3 | 期: | 4 | 來源出版物: | STAR protocols | 摘要: | Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/623997 | ISSN: | 26661667 | DOI: | 10.1016/j.xpro.2022.101678 |
顯示於: | 醫學系 |
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