Antihepatocarcinoma activity of lactic acid bacteria fermented Panax notoginseng
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
58
Journal Issue
15
Pages
8528-8534
Date Issued
2010
Author(s)
Abstract
Panax notoginseng was used as the medium for lactic acid bacteria fermentation to manufacture product with antihepatocarcinoma activity. The fermentation broth prepared in a 250 mL Erlenmeyer flask was found to possess antiproliferation activity against hepatoma Hep3B cells. At the dosage of 500 £gg/mL, the viability of hepatoma Hep3B cells was approximately 2.2%. When the fermentation was scaled up to a 6.6 L fermenter, it was found that the fermentation broth produced at 37 ¢XC for 2 days showed the highest antihepatoma activity. Animal study revealed that when Hep3B implanted SCID mice were treated with 1000 mg/kg BW/day of the fermentation broth, tumor volume and tumor weight were reduced approximately 60% as compared to the negative control group. HPLC analyses showed that saponins in P. notoginseng including notoginsenoside R1 and ginsenosides Rg1, Rb1, Rd, and Rh4 decreased, but ginsenosides Rh1 and Rg 3 increased during fermentation. LC-MS/MS revealed that the minor saponins ginsenoside F1, protopanaxatriol, and notoginseng R 2 also exist in the fermentation product. It appears that ginsenoside Rg3, ginsenoside Rh1, and protopanaxatriol are possibly responsible for the enhanced antihepatocarcinoma activity of the P. notoginseng fermentation broth. ? 2010 American Chemical Society.
Subjects
antihepatocarcinoma activity
fermentation
lactic acid bacteria
Panax notoginseng
saponins
SDGs
Other Subjects
Animal studies; antihepatocarcinoma activity; Erlenmeyer flasks; Fermentation broths; Fermentation products; Ginsenosides; HPLC analysis; Lactic acid bacteria; LC-MS/MS; Negative control; Panax notoginseng; saponins; Tumor volumes; Bacteriology; Body fluids; Lactic acid; Metabolites; Pharmacodynamics; Rhodium; Tumors; Fermentation; antineoplastic agent; herbaceous agent; lactic acid; animal; article; bacterium; chemistry; disease model; fermentation; hepatoblastoma; human; liver tumor; male; metabolism; microbiology; mouse; mouse mutant; Panax notoginseng; tumor cell line; Animals; Antineoplastic Agents, Phytogenic; Bacteria; Cell Line, Tumor; Disease Models, Animal; Drugs, Chinese Herbal; Fermentation; Hepatoblastoma; Humans; Lactic Acid; Liver Neoplasms; Male; Mice; Mice, SCID; Panax notoginseng; Animalia; Bacteria (microorganisms); Mus; Panax notoginseng
Type
journal article