https://scholars.lib.ntu.edu.tw/handle/123456789/413302
標題: | Antihepatocarcinoma activity of lactic acid bacteria fermented Panax notoginseng | 作者: | Lin Y.-W. Mou Y.-C. Su C.-C. Chiang B.-H. |
關鍵字: | antihepatocarcinoma activity;fermentation;lactic acid bacteria;Panax notoginseng;saponins | 公開日期: | 2010 | 卷: | 58 | 期: | 15 | 起(迄)頁: | 8528-8534 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | Panax notoginseng was used as the medium for lactic acid bacteria fermentation to manufacture product with antihepatocarcinoma activity. The fermentation broth prepared in a 250 mL Erlenmeyer flask was found to possess antiproliferation activity against hepatoma Hep3B cells. At the dosage of 500 £gg/mL, the viability of hepatoma Hep3B cells was approximately 2.2%. When the fermentation was scaled up to a 6.6 L fermenter, it was found that the fermentation broth produced at 37 ¢XC for 2 days showed the highest antihepatoma activity. Animal study revealed that when Hep3B implanted SCID mice were treated with 1000 mg/kg BW/day of the fermentation broth, tumor volume and tumor weight were reduced approximately 60% as compared to the negative control group. HPLC analyses showed that saponins in P. notoginseng including notoginsenoside R1 and ginsenosides Rg1, Rb1, Rd, and Rh4 decreased, but ginsenosides Rh1 and Rg 3 increased during fermentation. LC-MS/MS revealed that the minor saponins ginsenoside F1, protopanaxatriol, and notoginseng R 2 also exist in the fermentation product. It appears that ginsenoside Rg3, ginsenoside Rh1, and protopanaxatriol are possibly responsible for the enhanced antihepatocarcinoma activity of the P. notoginseng fermentation broth. ? 2010 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413302 | ISSN: | 00218561 | DOI: | 10.1021/jf101543k | SDG/關鍵字: | Animal studies; antihepatocarcinoma activity; Erlenmeyer flasks; Fermentation broths; Fermentation products; Ginsenosides; HPLC analysis; Lactic acid bacteria; LC-MS/MS; Negative control; Panax notoginseng; saponins; Tumor volumes; Bacteriology; Body fluids; Lactic acid; Metabolites; Pharmacodynamics; Rhodium; Tumors; Fermentation; antineoplastic agent; herbaceous agent; lactic acid; animal; article; bacterium; chemistry; disease model; fermentation; hepatoblastoma; human; liver tumor; male; metabolism; microbiology; mouse; mouse mutant; Panax notoginseng; tumor cell line; Animals; Antineoplastic Agents, Phytogenic; Bacteria; Cell Line, Tumor; Disease Models, Animal; Drugs, Chinese Herbal; Fermentation; Hepatoblastoma; Humans; Lactic Acid; Liver Neoplasms; Male; Mice; Mice, SCID; Panax notoginseng; Animalia; Bacteria (microorganisms); Mus; Panax notoginseng |
顯示於: | 食品科技研究所 |
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