|Title:||Antihepatocarcinoma activity of lactic acid bacteria fermented Panax notoginseng||Authors:||Lin Y.-W.
|Keywords:||antihepatocarcinoma activity;fermentation;lactic acid bacteria;Panax notoginseng;saponins||Issue Date:||2010||Journal Volume:||58||Journal Issue:||15||Start page/Pages:||8528-8534||Source:||Journal of Agricultural and Food Chemistry||Abstract:||
Panax notoginseng was used as the medium for lactic acid bacteria fermentation to manufacture product with antihepatocarcinoma activity. The fermentation broth prepared in a 250 mL Erlenmeyer flask was found to possess antiproliferation activity against hepatoma Hep3B cells. At the dosage of 500 £gg/mL, the viability of hepatoma Hep3B cells was approximately 2.2%. When the fermentation was scaled up to a 6.6 L fermenter, it was found that the fermentation broth produced at 37 ¢XC for 2 days showed the highest antihepatoma activity. Animal study revealed that when Hep3B implanted SCID mice were treated with 1000 mg/kg BW/day of the fermentation broth, tumor volume and tumor weight were reduced approximately 60% as compared to the negative control group. HPLC analyses showed that saponins in P. notoginseng including notoginsenoside R1 and ginsenosides Rg1, Rb1, Rd, and Rh4 decreased, but ginsenosides Rh1 and Rg 3 increased during fermentation. LC-MS/MS revealed that the minor saponins ginsenoside F1, protopanaxatriol, and notoginseng R 2 also exist in the fermentation product. It appears that ginsenoside Rg3, ginsenoside Rh1, and protopanaxatriol are possibly responsible for the enhanced antihepatocarcinoma activity of the P. notoginseng fermentation broth. ? 2010 American Chemical Society.
|Appears in Collections:||食品科技研究所|
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