A MICROFLUIDIC PLATFORM FOR UNDILUTED PLASMA SEPARATION AND ELECTROCHEMICAL DETECTION OF C-REACTIVE PROTEIN
Journal
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
ISBN
9781733419031
Date Issued
2021-01-01
Author(s)
Lai, Zhi Xuan
Abstract
We develop a miniaturized microfluidic platform integrating membrane-based, sedimentation-assisted plasma separation with cardiovascular disease (CVD) biomarker detection. The microfluidic platform can extract 177 μL plasma from 400 µL whole blood within 7 minutes and subsequently detect the biomarker in the undiluted plasma by an embedded electrochemical impedance spectroscopy (EIS) sensor. The EIS sensor successfully detected C-reactive protein (CRP), a cardiovascular disease biomarker, in a broad range from 1 ng/mL to 10 µg/mL. Highly integrated, the microfluidic platform exhibits great potential for point-of-care (POC) diagnostics.
Subjects
Cardiovascular Disease | Electrochemical Detection | Microfluidics | Whole Blood Processing
SDGs
Type
conference paper