Galectin-3 gene (LGALS3) +292C allele is a genetic predisposition factor for rheumatoid arthritis in Taiwan
Journal
Clinical Rheumatology
Journal Volume
30
Journal Issue
9
Pages
1227-1233
Date Issued
2011
Author(s)
Abstract
Galectin-3 is a beta-galactoside-binding lectin which is involved in modulating inflammation and apoptosis. Elevated expression of galectin-3 has been demonstrated in synovium of rheumatoid arthritis (RA). The aim of our study is to investigate the genetic polymorphisms of galectin-3 in association with RA. Polymorphisms of galectin-3 gene (LGALS3) were compared between 151 RA patients and 182 healthy subjects in Taiwan. Variants at two LGALS3 single nucleotide polymorphism (SNP) sites (rs4644 and rs4652, corresponding to LAGLS3 +191 and +292) were genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and sequence-specific oligonucleotide probe hybridization, respectively. The allelic carriage of LGALS3 +292C was increased in patients with RA (66.9% in RA vs. 52.7% in controls, odds ratio=1.8, 95% confidence interval=1.2-2.8, p=0.009). These results implicate that the genetic polymorphisms in galectin-3 gene may contribute to development of RA. ? 2011 Clinical Rheumatology.
SDGs
Other Subjects
alanine aminotransferase; aspartate aminotransferase; creatinine; galectin 3; methotrexate; tumor necrosis factor alpha inhibitor; adult; allele; article; blood sampling; controlled study; enzyme linked immunosorbent assay; female; gene frequency; gene identification; genetic association; genetic predisposition; genetic susceptibility; genetic variability; genotype; haplotype; human; hybridization; major clinical study; male; oligonucleotide probe; polymerase chain reaction; priority journal; protein expression; restriction fragment length polymorphism; rheumatoid arthritis; sequence specific oligonucleotide probe hybridization; single nucleotide polymorphism; Taiwan; Arthritis, Rheumatoid; Female; Galectin 3; Gene Frequency; Genetic Predisposition to Disease; Humans; Male; Middle Aged; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Taiwan
Type
journal article