Differential display screening for specific gene expression induced by dietary nonsteroidal estrogen
Journal
Molecular Reproduction and Development
Journal Volume
52
Journal Issue
2
Date Issued
1999-01-11
Author(s)
Abstract
The dietary phytoestrogen, daidzein, produced a biphasic response in cell proliferation of cultured, estrogen-responsive human breast carcinoma MCF-7 cells. Cell growth was stimulated at a daidzein concentration of 0.25 μg/ml whereas the addition of daidzein at concentrations >25 μg/ml significantly inhibited cell growth in a dose-dependent fashion, resulting in an IC50 value of 50 μg/ml. Upon exposure to 50 μg/ml of daidzein, cell morphology was severely altered, cell volume decreased, and condensation of the chromosomes was clearly noticeable. To identify genes whose expression were inhibited by daidzein, a differential display reverse transcriptase polymerase chain reaction assay (DD-RT-PCR) was performed and the cDNA fragments of several daidzein-regulated genes were visualized. The sequence of one of the cloned cDNA fragments that showed differential mRNA expression level in response to daidzein at a concentration of 50 μg/ml had a high homology with a cDNA expressed in fetal human brain, EST 06411.
Subjects
Fertility; Legume; Phytoestrogen
SDGs
Type
journal article