https://scholars.lib.ntu.edu.tw/handle/123456789/630674
標題: | Differential display screening for specific gene expression induced by dietary nonsteroidal estrogen | 作者: | JIH-TAY HSU Jean, Tzu Chao Chan, May Al Ying, Chingwen |
關鍵字: | Fertility; Legume; Phytoestrogen | 公開日期: | 11-一月-1999 | 卷: | 52 | 期: | 2 | 來源出版物: | Molecular Reproduction and Development | 摘要: | The dietary phytoestrogen, daidzein, produced a biphasic response in cell proliferation of cultured, estrogen-responsive human breast carcinoma MCF-7 cells. Cell growth was stimulated at a daidzein concentration of 0.25 μg/ml whereas the addition of daidzein at concentrations >25 μg/ml significantly inhibited cell growth in a dose-dependent fashion, resulting in an IC50 value of 50 μg/ml. Upon exposure to 50 μg/ml of daidzein, cell morphology was severely altered, cell volume decreased, and condensation of the chromosomes was clearly noticeable. To identify genes whose expression were inhibited by daidzein, a differential display reverse transcriptase polymerase chain reaction assay (DD-RT-PCR) was performed and the cDNA fragments of several daidzein-regulated genes were visualized. The sequence of one of the cloned cDNA fragments that showed differential mRNA expression level in response to daidzein at a concentration of 50 μg/ml had a high homology with a cDNA expressed in fetal human brain, EST 06411. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032923490&doi=10.1002%2f%28SICI%291098-2795%28199902%2952%3a2%3c141%3a%3aAID-MRD4%3e3.0.CO%3b2-V&partnerID=40&md5=a53a330f0d9be63046452877218ab4f6 https://scholars.lib.ntu.edu.tw/handle/123456789/630674 |
ISSN: | 1040452X | DOI: | 10.1002/(SICI)1098-2795(199902)52:2<141::AID-MRD4>3.0.CO;2-V |
顯示於: | 動物科學技術學系 |
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