Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus
Journal
Vaccines
Journal Volume
10
Journal Issue
2
Date Issued
2022
Author(s)
Su F.-J
Abstract
Photobacterium damselae subsp. damselae is a pathogen that mainly infects a variety of fish species. There are many antibiotic-resistant strains of Photobacterium damselae subsp. damselae. In a previously published article, we described the production method for a novel oral biofilm vaccine. In the study reported herein, we confirmed the protective effect of the oral biofilm vaccine against Photobacterium damselae subsp. damselae. Twenty-eight days after vaccination, phagocytosis increased by 256% relative to the control group. The mean albumin–globulin ratios of the vaccine groups were significantly lower than the mean albumin–globulin ratios of the control group. There were no significant intergroup differences in lysozyme activity. Mean IgM titers were significantly higher in the vaccine group than in the control group. There was a significant upregulation of the TLR 3, IL-1β, and IL-8 genes in the spleen 28 days after vaccination. The cumulative mortality of the control fish was 84% after challenging fish with the Photobacterium damselae subsp. damselae, while the cumulative mortality of the oral biofilm vaccine (PBV) group was 32%, which was significantly higher than those of the whole-cell vaccine (PWV) and chitosan particle (CP) groups. There is minimal published research on the prevention and treatment of Photobacterium damselae subsp. damselae infection; therefore, this oral biofilm vaccine may represent a new method to fill this gap. ? 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Epinephelus lanceolatus
Oral biofilm vaccine
Photobacterium damselae subsp. damselae
cell-based vaccine
cytokine
gamma interferon
immunoglobulin A
immunoglobulin enhancer binding protein
immunoglobulin M
interleukin 10
interleukin 1beta
interleukin 4
interleukin 6
interleukin 8
lysozyme
RNA 16S
toll like receptor 3
tumor necrosis factor
affinity chromatography
animal experiment
antibiotic resistance
antibody response
aquaculture
Article
bacterial infection
bacterial strain
bacteriophage
biofilm
cell viability
controlled study
enzyme activity
enzyme linked immunosorbent assay
enzyme linked immunospot assay
Epinephelus
Escherichia coli
fish
gene expression
humoral immunity
immune response
innate immunity
intestine flora
Lactobacillus casei
Lactobacillus plantarum
Lactococcus garvieae
mortality
nonhuman
phagocytosis
Photobacterium
Photobacterium damselae
polyacrylamide gel electrophoresis
polymerase chain reaction
real time polymerase chain reaction
scanning electron microscopy
surface property
survival rate
upregulation
vaccination
SDGs
Type
journal article