https://scholars.lib.ntu.edu.tw/handle/123456789/605980
標題: | Protective Efficacy of Novel Oral Biofilm Vaccines against Photobacterium damselae subsp. damselae Infection in Giant Grouper, Epinephelus lanceolatus | 作者: | Su F.-J MEEI-MEI CHEN |
關鍵字: | Epinephelus lanceolatus;Oral biofilm vaccine;Photobacterium damselae subsp. damselae;cell-based vaccine;cytokine;gamma interferon;immunoglobulin A;immunoglobulin enhancer binding protein;immunoglobulin M;interleukin 10;interleukin 1beta;interleukin 4;interleukin 6;interleukin 8;lysozyme;RNA 16S;toll like receptor 3;tumor necrosis factor;affinity chromatography;animal experiment;antibiotic resistance;antibody response;aquaculture;Article;bacterial infection;bacterial strain;bacteriophage;biofilm;cell viability;controlled study;enzyme activity;enzyme linked immunosorbent assay;enzyme linked immunospot assay;Epinephelus;Escherichia coli;fish;gene expression;humoral immunity;immune response;innate immunity;intestine flora;Lactobacillus casei;Lactobacillus plantarum;Lactococcus garvieae;mortality;nonhuman;phagocytosis;Photobacterium;Photobacterium damselae;polyacrylamide gel electrophoresis;polymerase chain reaction;real time polymerase chain reaction;scanning electron microscopy;surface property;survival rate;upregulation;vaccination | 公開日期: | 2022 | 卷: | 10 | 期: | 2 | 來源出版物: | Vaccines | 摘要: | Photobacterium damselae subsp. damselae is a pathogen that mainly infects a variety of fish species. There are many antibiotic-resistant strains of Photobacterium damselae subsp. damselae. In a previously published article, we described the production method for a novel oral biofilm vaccine. In the study reported herein, we confirmed the protective effect of the oral biofilm vaccine against Photobacterium damselae subsp. damselae. Twenty-eight days after vaccination, phagocytosis increased by 256% relative to the control group. The mean albumin–globulin ratios of the vaccine groups were significantly lower than the mean albumin–globulin ratios of the control group. There were no significant intergroup differences in lysozyme activity. Mean IgM titers were significantly higher in the vaccine group than in the control group. There was a significant upregulation of the TLR 3, IL-1β, and IL-8 genes in the spleen 28 days after vaccination. The cumulative mortality of the control fish was 84% after challenging fish with the Photobacterium damselae subsp. damselae, while the cumulative mortality of the oral biofilm vaccine (PBV) group was 32%, which was significantly higher than those of the whole-cell vaccine (PWV) and chitosan particle (CP) groups. There is minimal published research on the prevention and treatment of Photobacterium damselae subsp. damselae infection; therefore, this oral biofilm vaccine may represent a new method to fill this gap. ? 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123999993&doi=10.3390%2fvaccines10020207&partnerID=40&md5=b2c069841f3de62a460c4200c4c74210 https://scholars.lib.ntu.edu.tw/handle/123456789/605980 |
ISSN: | 2076393X | DOI: | 10.3390/vaccines10020207 |
顯示於: | 獸醫學系 |
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