Planarian mobility assay as a simple alternative for rapid screening aquatic acute toxicity of personal care products
Journal
Fresenius Environmental Bulletin
Journal Volume
29
Journal Issue
5
Pages
3362-3369
Date Issued
2020
Author(s)
Abstract
The effects of 25 chemicals used in personal care products, including benzophenone-type UV filters, paraben preservatives, and surfactants, on freshwater planarian locomotion were examined using an automated video tracking system during the second 5-min period exposure. The slope of the linear regression for second 5-min EC5o and 24-h median lethal concentration (LC50 ) was 0.9904. The second 5-min EC50 values of planarian mobility were similar to their corresponding 24-h LC50 values. The results suggest that changes in mobility velocity in planarians can serve as a rapid acute aquatic toxicity indicator. Moreover, planarian mobility assay can be a useful tool in preliminary evaluation of water samples and in deciding the timely precautionary measures required to prevent episodic water pollution, even in the absence of acute aquatic toxicity information. Based on the result of this study, planarian mobility assay provides great advantages to promote 3Rs principle in toxicology study, by replacing vertebrates to invertebrates, reducing numbers of experimental animals used, and refining animals by greatly increased survival and recovery. © 2020 Parlar Scientific Publications. All rights reserved.
SDGs
Other Subjects
bioassay; concentration (composition); locomotion; mobility; paraben; PPCP; regression analysis; survival; toxicity; toxicology; tracking; water pollution; Animalia; Invertebrata; Planaria; Turbellaria; Vertebrata
Type
journal article