https://scholars.lib.ntu.edu.tw/handle/123456789/137009
標題: | Azetidine-induced Accumulation of Class I Small Heat Shock Proteins in the Soluble Fraction Provides Thermotolerance in Soybean Seedlings | 作者: | Jinn, Tsung-Luo Chiu, Chi-Chou Song, Wei-Wen Chen, Yih-Ming Lin, Chu-Yung |
關鍵字: | Amino acid analog; Azetidine; Glycine max; Heat shock; Plant class I sHSPs; Thermotolerance | 公開日期: | 2004 | 卷: | 45 | 期: | 12 | 起(迄)頁: | 1759-1767 | 來源出版物: | Plant and Cell Physiology | 摘要: | Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40°C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45°C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-12444274952&doi=10.1093%2fpcp%2fpch193&partnerID=40&md5=ee33c088e96241f3cb9d949ce933d807 http://ntur.lib.ntu.edu.tw//handle/246246/162130 |
ISSN: | 00320781 | DOI: | 10.1093/pcp/pch193 | SDG/關鍵字: | Glycine max |
顯示於: | 生命科學系 |
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