https://scholars.lib.ntu.edu.tw/handle/123456789/152177
標題: | 總計畫:超音波釋控微脂體科技於腫瘤預防診斷治療之研究 Ultrasound Assisted Liposomal Cancer Therapy |
作者: | 李百祺 | 關鍵字: | 超音波;微氣泡對比劑;微脂體;穴蝕化;癌症治療;藥物輸送;ultrasound;contrast microbubbles;liposome;cavitation;cancer treatment;drug delivery | 公開日期: | 31-七月-2004 | 出版社: | 臺北市:國立臺灣大學電機工程學系暨研究所 | 摘要: | 本整合型計畫之主要目標,為結合超音波科技及靶向傳輸原理,利用微脂體之穴蝕化效 應與藥物傳輸能力,發展一腫瘤早期診斷與治療之創新技術,並應用於國內常見之肝癌、 胃癌及大腸直腸癌等相關疾病。以微脂體為主之藥物傳送系統,在近年來已獲得普遍之重 視。此類系統亦可以結合靶向技術,將抗癌藥物直接作用於癌細胞,提高靶區之藥物濃度, 卻不影響正常細胞區。除了藥物輸送方式之外,本計畫亦研究在微脂體中包覆氣體以構成 穴蝕化之核心,配合最佳化之超音波物理參數,有效利用整流擴散原理,提昇穴蝕化效率 及產生氣泡破壞,以達到治療癌症之效果。此外,本計畫之另一目標為應用活體微透析探 針及微光纖系統,於腫瘤原位判定微脂體靶向定位傳輸效率,並依此針對超音波物理參數 及微脂體表面傳輸定位抗體及修飾標定做最佳組合。本計畫在前九個月先期研究中,已完 成了下列數項工作,成功評估含藥微脂粒及瞬間穴蝕效應對癌細胞和腫瘤組織的抗癌作 用,為後續研究建立一良好之基礎與可行性之驗證。 - 超音波穴蝕效應之有效誘發與偵測 - 穴蝕效應實驗系統建立及數值模擬器之開發 - 包覆空氣之微脂體微氣泡之配方研究與製程確認 - 建立微光纖及微透析之活體偵測分析系統及氧濃度分佈造影系統 - 含doxorubicin 之微脂粒的抗癌活性研究 - 可穩定表現GFP 之MKN45 胃癌細胞培養 - 利用MKN45 細胞建立之癌細胞株原位植入小裸鼠胃體內的動物實驗模式。 在後續三年之研究中,總計畫將持續負責整合各子計畫之工作,以探討相關技術在組織與 細胞中之療效,並針對皮下異種移植腫瘤與原位異種移植腫瘤之抗癌作用進行後續之探討。 The prevalence of cancer disease is a major health problem worldwide. Cancer has been the leading cause of death over the past two decades in Taiwan. This project focuses on the development of a system involving targeting delivery for liposomal air-bubbles and ultrasound-assisted release of the drug carried in liposome particles. The primary clinical interests are stomach cancer and colon cancer diseases. In addition to drug delivery using liposome, the air encapsulated in liposomes can also be used to enhance the contrast for ultrasound images, in addition to the generation of cavitation induced by ultrasound wave. Tumor cells death will be concomitant with the cavitation. Another aspect of the project aims at the development of two micro-systems, one is the microdialysis perfusion system for the evaluation of liposomal targeting efficiency, and the other is the microlight-guide system for evaluation of tumor functional images. In addition, a non-invasive system using the oxygen quenched infrared phosphorescence will be constructed to obtain the two dimensional tissue oxygen concentration profile. In the initial phase of the project (i.e., first 9 months), the project has addressed the following issues: - Effective induction and detection of acoustic cavitation - Setup of an experimental system and simulator for analyzing cavitation - Process and recipe for producing air encapsulated liposome microbubbles - Development of the two micro-systems - Study of the therapeutic effects of doxorubicin encapsulated liposome particles - Cell culture for MKN45 with GFP With the solid progress of the initial phase project, we will continue to coordinate and integrate the results from sub-projects, and to evaluate the anti-tumoral efficacy of cavitation effects of the air bubbles-loaded liposomal formulation and targeted drug delivery in cancer cells and cultured cancer cells. The project will also move on to cancer therapy in the tumor xenograft model in animals and in the in situ implanted cancers. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/7997 | 其他識別: | 922218E002043 | Rights: | 國立臺灣大學電機工程學系暨研究所 |
顯示於: | 電機工程學系 |
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922218E002043.pdf | 102.41 kB | Adobe PDF | 檢視/開啟 |
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