https://scholars.lib.ntu.edu.tw/handle/123456789/160050
標題: | Polyclonal Anticardiolipin Antibodies Purified from Sera of Patients with Active Systemic Lupus Erythematosus Induce Apoptosis of the Cultured Glomerular Mesangial Cells | 作者: | TSAI, CHANG-YU YU, CHIA-LI |
關鍵字: | anticardiolipin antibodies;apoptosis;glomerular mesangial cell;lupus nephritis;ANTI-DNA ANTIBODIES;ANTIPHOSPHOLIPID ANTIBODIES | 公開日期: | 2000 | 卷: | v.29 | 期: | n.6 | 起(迄)頁: | 370-379 | 來源出版物: | SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 摘要: | Objective: To test the effect of anticardiolipin antibodies( aCL) on cultured glomerular mesangial cells with regard to their expression of apoptosis-related genes. Methods: aCL purified from active lupus sera by cardiolipin micelles were incubated with cultured rodent mesangial cells ( RMC). Morphological changes of the RMC were observed. The genomic DNA was extracted for the detection of apoptosis. The total cell RNA was extracted for detection of Fas, c-myc, p53, and bcl-2 transcripts by reverse transcription-polymerase chain reaction. Results: aCL (100 GPL-U/0.1 mg protein/ml) bound to RMC more prominent than human IgG (100 mug/ml). The antibodies suppressed RMC proliferation in a dose-dependent manner. The RMC were undergoing apoptosis as evidenced by morphologic changes, fluorescein-annexin V staining and appearance of nucleosome-sized DNA fragments. RMC spontaneously express p53 and c-myc but not Fas or bcl-2. aCL (100 GPL-U/ml) enhanced the expression of Fas but not other apoptosis- related genes and suppressed the intracellular tyrosine phosphorylation. Conclusions: Binding of aCL can induce apoptosis of the RMC. The aCL may be implicated in the pathogenesis of lupus nephritis. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95053 |
顯示於: | 分子醫學研究所 |
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