https://scholars.lib.ntu.edu.tw/handle/123456789/162623
Title: | 哺乳動物腺核三磷酸感受性鉀離子通道晶體繞射結構性質之研究 Involvement of a novel C-terminal kinase domain of Kir6.2 in the K-ATP channel rundown reactivation |
Authors: | 樓國隆 Lou, Kuo-Long |
Keywords: | Channel gating;3D homology modeling;Kinase domain;Kir6.2;Rundown reactivation | Issue Date: | 2001 | Publisher: | 臺北市:國立臺灣大學醫學院口腔生物科學研究所 | Start page/Pages: | 20-25 | Source: | J Mol Model 2001(7) | Abstract: | Rundown is a generally encountered problem while recording KATP channel activity with inside-out patches. No assigned structural fragment related to this mechanism has yet been derived from any of the functional analyses performed. Therefore, based on a combined sequence and secondary structure alignment against known crystal structure of segments from closely related proteins, we propose here the three-dimensional structural model of an intracellular C-terminal domain of the Kir6.2 subunit in KATP channels. An E. coli CMPkinase was suggested as template for the model building. The subdomain arrangement of this novel kinase domain and the structural correlation for UDP-docking are described. With structural-functional interpretation, we conclude that the reactivation of KATP channel rundown by MgATP or UDP is very possibly regulated by this intracellular kinase domain at the C-terminus of Kir6.2 subunit in KATP channels. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/22658 | Other Identifiers: | 892320B002234 | Rights: | 國立臺灣大學醫學院口腔生物科學研究所 |
Appears in Collections: | 口腔生物科學研究所 |
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892320B002234.pdf | 163.8 kB | Adobe PDF | View/Open |
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