https://scholars.lib.ntu.edu.tw/handle/123456789/183989
標題: | Anti-metastatic activities of Antrodia camphorata against human breast cancer cells mediated through suppression of the MAPK signaling pathway | 作者: | Yang, Hsin-Ling Kuo, Yueh-Hsiung Tsai, Ching-Tsan Huang, Yi-Ting Chen, Ssu-Ching Chang, Hsueh-Wei Lin, Elong Lin, Wen-Hsin Hseu, You-Cheng |
關鍵字: | Antrodia camphorata; Breast cancer; Chinese herb; Matrix metalloproteinase; Metastasis; Nuclear factor κB | 公開日期: | 2011 | 起(迄)頁: | 290-298 | 來源出版物: | Food and Chemical Toxicology | 摘要: | The fermented culture broth of Antrodia camphorata (A. camphorata) has been shown to promote cell cycle arrest and apoptosis of human estrogen-nonresponsive MDA-MB-231 cells. Herein, we demonstrate that non-cytotoxic concentrations (20-80. μg/mL) of A. camphorata markedly inhibited the invasion/migration of highly metastatic MDA-MB-231 cells as shown by an in vitro transwell and a wound-healing repair assay. The results of a gelatin zymography assay showed that A. camphorata suppressed the activity of matrix metalloproteinase (MMP)-9 and urokinase plasminogen activator (uPA). Western blot results demonstrated that treatment with A. camphorata decreased the expression of MMP-9, MMP-2, uPA, uPA receptor (uPAR) and vascular endothelial growth factor (VEGF); while the expression of the endogenous inhibitors of these proteins, i.e., tissue inhibitors of MMP (TIMP-1 and TIMP-2), and plasminogen activator inhibitor (PAI)-1, increased. Further investigation revealed that A. camphorata suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. camphorata treatment also led to a dose-dependent inhibition on NF-κB binding and activation. This is the first report confirming the anti-metastatic activity of this potentially beneficial mushroom against human breast cancer. ? 2010 Elsevier Ltd. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/239255 | DOI: | 10.1016/j.fct.2010.10.031 | SDG/關鍵字: | gelatinase A; gelatinase B; immunoglobulin enhancer binding protein; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase p38; plasminogen activator inhibitor; stress activated protein kinase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; urokinase; urokinase receptor; vasculotropin; Antrodia camphorata; apoptosis; article; assay; breast cancer; cancer cell; cell cycle arrest; cell invasion; cell migration; concentration response; controlled study; enzyme repression; fungus culture; human; human cell; in vitro study; metastasis inhibition; mushroom; protein expression; signal transduction; Western blotting; zymography; Antrodia; Blotting, Western; Breast Neoplasms; Cell Line, Tumor; Humans; MAP Kinase Signaling System; Matrix Metalloproteinases; Neoplasm Metastasis; Phosphorylation; Urokinase-Type Plasminogen Activator; Vascular Endothelial Growth Factor A; Basidiomycota; Taiwanofungus camphoratus |
顯示於: | 化學系 |
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