https://scholars.lib.ntu.edu.tw/handle/123456789/188196
Title: | 人類上皮脂肪酸結合蛋白基因與肺癌細胞轉移能力之相關性研究 | Authors: | 鄭之勛 | Keywords: | 癌細胞轉移;微陣列;人類上皮脂肪酸結合蛋白;metastasis;microarray;human fatty acid- binding protein | Issue Date: | 2003 | Publisher: | 臺北市:國立臺灣大學醫學院內科 | Abstract: | 癌細胞之轉移為癌症病患的主要死因之一,發現 癌細胞轉移相關基因仍為目前癌症研究的一大課題。 在先前的研究中,利用微陣列及肺癌轉移模式細胞株 (CL1 系列),已經能成功地篩選出許多與癌轉移相關的 基因,其中部份已定性出為新的癌轉移相關基因,甚 至發現其具臨床轉移上的相關性。本研究計畫所要研 究的標的為人類上皮脂肪酸結合蛋白基因FABP5 。經 由微陣列篩選檢驗,發現此轉譯此蛋白質之基因的 mRNA 在肺癌腫瘤細胞株的表現,轉移能力較低的細 胞株表現較少,並隨著在轉移能力的增強而有逐步且 明顯的增加。另由聚合脢鏈反應之結果,亦顯示此基 因之表現與肺癌腫瘤細胞株系列之轉移能力可能有正 相關性。本研究計畫中,我們由轉移能力較強的人類 肺癌細胞株CL1-5 中選殖FABP5 基因並導入較不具轉 移能力的細胞株CL1-0 中以增加其FABP5 的表現量, 藉以產生穩定的選殖細胞株,並以此細胞株進行人體 外之細胞侵襲能力檢定、細胞生長能力鑑定以及轉移 能力檢定,以分析此基因在癌轉移促進的能力。我們 預期在較不具轉移能力的細胞株CL1-0 中過度增加其 FABP5 的表現量會導致細胞侵襲能力與轉移能力的增加。 Metastasis remains the major cause of death from cancer. It may be of importance that discovering novel genes may elucidate the detailed mechanism of cancer metastasis. In the previous studies, by using cDNA microarray, we have identified several invasion/ metast- asis-related genes from established lung cancer cell model of metastasis and some them have been considered as novel genes in invasion/metastasis and correlated closely with clinical metastasis. The target of this study is one of the potential metastasis-related genes, human epidermal fatty acid-binding protein (E-FABP) gene FABP5. The results from microarray confirmed that FABP5 mRNA expression was in a lower level in less metastatic cell line such as CL1-0 and was greatly increased in the highly metastatic cell lines such as CL1-5 and CL1-5-F4. Reverse-transcriptase polymerase chain reaction (RT-PCR) also showed that the expression of FABP5 mRNA increase was correlated with the metastatic ability of human lung cancer cell lines. In this proposed study, we performed molecular cloning of the 437-base paired, full length cDNA of FABP5 from highly metastatic CL1-5 cell line onto a mammalian expression plasmid vector, which was then be transfected into a less metastatic human lung adenocarcinoma cell line, CL1-0. The cell line was analyzed the metastasis- promoting function of FABP5 by in vitro invasion assay, cell growth assay, and in vitro metastasis assay. We expect that overexpression of FABP5 in the less metastatic human lung adenocarcinoma cell line will enhance their activity of invasiveness and metastasis. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23595 | Other Identifiers: | 912314B002229 | Rights: | 國立臺灣大學醫學院內科 |
Appears in Collections: | 醫學系 |
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912314B002229.pdf | 119.98 kB | Adobe PDF | View/Open |
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