https://scholars.lib.ntu.edu.tw/handle/123456789/195003
標題: | Identification and Expression of Pen C 2, a Novel Allergen from Penicillium Citrinum | 作者: | CHOW, Lu-Ping SU, Ning-Yuan YU, Chia-Jung CHIANG, Bor-Luen SHEN, Horng-Der |
關鍵字: | allergy;cDNA cloning;IgE-binding activity;mould allergens;vacuolar serine protease;GROUP-III ALLERGEN | 公開日期: | 1999 | 卷: | v.341 | 期: | n.PART1 | 起(迄)頁: | 51-59 | 來源出版物: | BIOCHEMICAL JOURNAL | 摘要: | The mould genus, Penicillium, is known to be a significant source of environmental aero-allergens. One important allergen from Penicillium citrinum, Pen c 2, has been identified by means of two-dimensional immunoblotting using IgE-containing patients' sera. This novel allergen was cloned, sequenced and expressed in Escherichia toll. The cloned cDNA encodes a large 457-amino acid protein precursor containing a 16-amino acid signal peptide, a 120-amino acid propeptide and the 321-amino acid mature protein. Comparison of the Pen c 2 sequence with known protein sequences revealed shared high sequence similarities with two vacuolar serine proteases from Aspergillus niger and Saccharomyces cerevisiae. Asp- 36, His-78 and Ser-244 were found to constitute the catalytic triad of the 39-kDa Pen c 2, The DNA coding for Pen c 2 was cloned into vector PQE-30 and expressed in E. coli as a His-tag fusion protein that bound serum IgE from Penicillium-allergic patients on immunoblots, Recombinant Pen c 2 could therefore be used effectively for diagnosis and also potentially for the treatment of mould-derived allergic disorders. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94896 |
顯示於: | 醫學系 |
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