Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients
Journal
Journal of Medical Virology
Journal Volume
64
Journal Issue
1
Pages
51-57
Date Issued
2001
Author(s)
Liu, Mei-Ying
Hsu, Shih-Mei
Fong, Chin-Chu
Chen, Chien-Jen
Chen, I-How
Yang, Czau-Siung
Chen, Jen-Yang
Abstract
EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals. ? 2001 Wiley-Liss, Inc.
Subjects
Antibodies; EBNA-1; NPC
SDGs
Other Subjects
cell nucleus antigen; deoxyribonuclease; EBNA 1 protein; Epstein Barr virus antigen; glutathione transferase; unclassified drug; virus capsid antigen; article; cancer diagnosis; cancer screening; controlled study; diagnostic accuracy; diagnostic procedure; enzyme linked immunosorbent assay; Epstein Barr virus; human; nasopharynx carcinoma; screening test; serodiagnosis; Antibodies, Viral; Antigens, Viral; Biopsy; Capsid Proteins; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Epitopes; Epstein-Barr Virus Nuclear Antigens; Escherichia coli; Humans; Immunoglobulin A; Matched-Pair Analysis; Nasopharyngeal Neoplasms; Neutralization Tests; Plasmids; Recombinant Fusion Proteins; Sensitivity and Specificity; Serologic Tests; Taiwan
Type
journal article