https://scholars.lib.ntu.edu.tw/handle/123456789/349005
Title: | Growth inhibition in Chinese cabbage (Brassica rapa var. chinensis) growth exposed to di-n-butyl phthalate | Authors: | Liao, C.-S. JUI-HUNG YEN Wang, Y.-S. |
Keywords: | Chinese cabbage; Di-n-butyl phthalate; Endocrine disruptor; Hydroponic methods; Proteomics | Issue Date: | 2009 | Journal Volume: | 163 | Journal Issue: | 2-3 | Start page/Pages: | 625-631 | Source: | Journal of Hazardous Materials | Abstract: | The toxicity and effects of di-n-butyl phthalate (DBP), an endocrine disruptor, on the growth of Chinese cabbage (Brassica rapa var. chinensis) were studied. Etiolation occurred on leaves of Chinese cabbage plant treated with 50 mg/L of DBP for 42 d. DBP even below 1 mg/L had a significant effect on the concentration of chlorophyll in Chinese cabbage and the biomass showed a severe decrease under treatment with more than 30 mg/L of DBP. At a concentration below 1 mg/L of DBP, no significant difference in accumulation was found, but treatments with concentration exceeding 10, 30, 50 and 100 mg/L all resulted in significant accumulation of DBP. Six protein spots extracted from leaf tissue of DBP-treated Chinese cabbage displaying a differential expression are shown in 2-DE maps. According to proteome level studies, three protein spots were found to increase and were identified, respectively, as acyl-[acyl-carrier-protein] desaturase (acyl-ACP desaturase), root phototropism protein 3 (RPT3) and ferredoxin-nitrite reductase (Fd-NiR). The other three protein spots were found to decrease and were identified respectively as dihydroflavonol-4-reductase (DFR), aminoacyl-tRNA synthetase (aaRS) and ATP synthase subunit beta. The key finding is that the other closely related plant, Bok choy (Brassica rapa subsp. chinensis), the subspecies of Chinese cabbage, respond differently to the same chemicals. © 2008 Elsevier B.V. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-59849106051&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/349005 |
DOI: | 10.1016/j.jhazmat.2008.07.025 | SDG/Keyword: | Chinese cabbage; Di-n-butyl phthalate; Endocrine disruptor; Hydroponic methods; Proteomics; Amino acids; Biomass; Chlorophyll; Growth (materials); Molecular biology; Porphyrins; Proteins; Renewable energy resources; Value engineering; Esters; bioaccumulation; chlorophyll; dicotyledon; ecotoxicology; endocrine disruptor; enzyme activity; growth rate; hydroponics; inhibition; phthalate; phytomass; protein; proteomics; Brassica rapa; Dibutyl Phthalate; Dose-Response Relationship, Drug; Endocrine Disruptors; Enzymes; Plant Leaves; Plant Proteins; Plasticizers; Proteomics; Brassica rapa; Brassica rapa subsp. chinensis; Brassica rapa subsp. pekinensis |
Appears in Collections: | 農業化學系 |
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