https://scholars.lib.ntu.edu.tw/handle/123456789/350636
Title: | Diosgenin attenuates allergen-induced intestinal inflammation and IgE production in a murine model of food allergy | Authors: | Huang, C.-H. Ku, C.-Y. TONG-RONG JAN |
Keywords: | Diosgenin; Food allergy; IgE; Mast cells; Ovalbumin | Issue Date: | 2009 | Journal Volume: | 75 | Journal Issue: | 12 | Start page/Pages: | 1300-1305 | Source: | Planta Medica | Abstract: | Diosgenin, the major sapogenin contained in the Chinese yam, has recently been shown to promote systemic T helper 1-type immunity in a murine model of airway hypersensitivity. In this study, we hypothesized that diosgenin might be effective in modulating food allergy. BALB/c mice were either left untreated (na?ve; NA) or administered daily with vehicle (VH; olive oil) and/or diosgenin (100 or 200mg/kg) by gavage throughout the experiment. Except for the NA group, the mice were sensitized with ovalbumin (OVA) and repeatedly challenged with intragastric OVA to induce intestinal allergic responses. Diosgenin demonstrated a suppressive effect on the intestinal inflammation, including the occurrence of diarrhea, the infiltration and degranulation of mast cells, and the presence of mucin-containing goblet cells in the duodenum. A protective effect by diosgenin on reducing the crypt depth of the intestine was also observed in OVA-sensitized and challenged mice. Furthermore, the serum production of OVA-specific IgE, and the total IgE was suppressed. In contrast, OVA-specific IgG2a was enhanced by diosgenin treatment in OVA-sensitized mice. These results demonstrated the in vivo anti-allergic activity of diosgenin, which is associated with the suppression of IgE production and mast cell infiltration and degranulation. ? Georg Thieme Verlag KG Stuttgart. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-70449441241&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/350636 |
DOI: | 10.1055/s-0029-1185578 | SDG/Keyword: | diosgenin; food allergen; immunoglobulin E; immunoglobulin G2a; ovalbumin; allergic reaction; animal experiment; animal model; article; Bagg albino mouse; cell infiltration; controlled study; degranulation; diarrhea; enteritis; feeding; food allergy; goblet cell; immunoglobulin production; immunomodulation; in vivo study; male; mast cell; mouse; nonhuman; Allergens; Animals; Diosgenin; Disease Models, Animal; Food Hypersensitivity; Immunoglobulin E; Male; Mice; Mice, Inbred BALB C; Dioscorea polystachya; Murinae; Mus |
Appears in Collections: | 獸醫學系 |
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