https://scholars.lib.ntu.edu.tw/handle/123456789/365348
Title: | Adjuvant effect of liposome in chicken result from induction of nitric oxide | Authors: | Lin, Y.-F. Deng, M.-C. Tseng, L.-P. Jiang, P.-R. TONG-RONG JAN Hsieh, F.-I. Liu, D.-Z. |
Issue Date: | 2011 | Journal Volume: | 6 | Journal Issue: | 1 | Source: | Biomedical Materials | Abstract: | Intranasal delivery of liposome-encapsulated inactivated Newcastle Disease virus (NDV) is known to be an effective vaccine for inducing immunity in the respiratory tract from our previous reports. Four-week-old specific pathogen-free chickens were intranasally immunized with NDV entrapped in phosphatidylcholine-liposomes (PC-Lip). The mucosal levels of anti-NDV s-immunoglobulin A (IgA), serum IgG, a high hemagglutination inhibition titer (1:640), and the high survival rate with the PC-Lip vaccine were comparable to those of our previous report. The immune mechanisms of the PC-Lip adjuvant were determined by in vitro cellular experiments using the NO production of chicken spleen macrophages. The most important finding of this study was proving that macrophages were stimulated by PC-Lip via the extracellular regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB activation pathways. This finding may be useful for developing potent mucosal vaccine delivery systems in the future. ? 2011 IOP Publishing Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79551495396&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/365348 |
DOI: | 10.1088/1748-6041/6/1/015011 | SDG/Keyword: | Animals; Liposomes; Macrophages; Nitric oxide; Phospholipids; Viruses; Activation pathway; Extracellular-regulated kinase; Immune mechanism; In-vitro; Intranasal; Mucosal vaccine; Nuclear factors; Phosphatidylcholine; Respiratory tract; Survival rate; Vaccines; I kappa B; immunoglobulin A; immunoglobulin G; liposome; mitogen activated protein kinase 1; mitogen activated protein kinase 3; nitric oxide; phosphatidylcholine liposome vaccine; unclassified drug; virus vaccine; immunoglobulin enhancer binding protein; immunological adjuvant; liposome; phosphatidylcholine; virus vaccine; animal experiment; animal model; article; chicken; drug delivery system; immune response; in vitro study; macrophage; Newcastle disease; nonhuman; animal; biosynthesis; evaluation; immunology; intranasal drug administration; metabolism; mucosal immunity; Newcastle disease paramyxovirus; signal transduction; Th2 cell; Gallus gallus; Newcastle disease virus; Adjuvants, Immunologic; Administration, Intranasal; Animals; Chickens; Drug Delivery Systems; Immunity, Mucosal; Lecithins; Liposomes; Macrophages; MAP Kinase Signaling System; Newcastle disease virus; NF-kappa B; Nitric Oxide; Signal Transduction; Th2 Cells; Viral Vaccines |
Appears in Collections: | 獸醫學系 |
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