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  4. A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan
 
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A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan

Journal
Journal of Virological Methods
Journal Volume
191
Journal Issue
2
Pages
122-127
Date Issued
2013
Author(s)
Yang, Y.-C.
Yang-Chih Shih, D.
MONG-HSUN TSAI  
Cheng, C.-H.
Cheng, H.-F.
Lo, C.-F.
Wang, D.-Y.
DOI
10.1016/j.jviromet.2013.04.002
URI
http://www.scopus.com/inward/record.url?eid=2-s2.0-84878237417&partnerID=MN8TOARS
http://scholars.lib.ntu.edu.tw/handle/123456789/380685
Abstract
The World Health Organization (WHO) International Standard (IS) for human immunodeficiency virus type 1 (HIV-1) RNA is only available in limited amounts. It is critical to use the most common HIV-1 genotype as source for secondary standards, e.g. a National Standard for Taiwan. The objective of this study was to establish the first National Standard for HIV-1 RNA NAT assays in Taiwan. A collaborative study, including eleven laboratories from five different countries, was carried out to establish the HIV-1 RNA National Standard by calibration, in International Units (IU), against the WHO HIV-1 RNA IS. The HIV-1 RNA content for the candidate was quantitated by each laboratory in three independent assays and the results were collected and analyzed statistically. Overall, a high level of agreement among results was achieved from different laboratories. In addition, the stability study indicated that the candidate was stable for 24 months at -80 ± 5. °C. In conclusion, the candidate standard was established as the first National Standard for HIV-1 RNA for use in NAT assays in Taiwan. The standard is intended to be used for the quality control of HIV-1 NAT assays and as a quantitative reference material for HIV-1 NAT assays. ? 2013 Elsevier B.V.
Subjects
Human immunodeficiency virus type 1 (HIV-1); Nucleic acid amplification techniques (NAT); Standard
SDGs

[SDGs]SDG3

Other Subjects
article; bioassay; clinical laboratory; clinical study; controlled study; genotype; health care quality; human; Human immunodeficiency virus 1; nonhuman; nucleic acid amplification; priority journal; quality control; quantitative analysis; reference value; standard; Taiwan; HIV Infections; HIV-1; Humans; International Cooperation; Nucleic Acid Amplification Techniques; Reference Standards; RNA, Viral; Taiwan; Viral Load
Type
journal article

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