https://scholars.lib.ntu.edu.tw/handle/123456789/380685
標題: | A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan | 作者: | Yang, Y.-C. Yang-Chih Shih, D. MONG-HSUN TSAI Cheng, C.-H. Cheng, H.-F. Lo, C.-F. Wang, D.-Y. |
關鍵字: | Human immunodeficiency virus type 1 (HIV-1); Nucleic acid amplification techniques (NAT); Standard | 公開日期: | 2013 | 卷: | 191 | 期: | 2 | 起(迄)頁: | 122-127 | 來源出版物: | Journal of Virological Methods | 摘要: | The World Health Organization (WHO) International Standard (IS) for human immunodeficiency virus type 1 (HIV-1) RNA is only available in limited amounts. It is critical to use the most common HIV-1 genotype as source for secondary standards, e.g. a National Standard for Taiwan. The objective of this study was to establish the first National Standard for HIV-1 RNA NAT assays in Taiwan. A collaborative study, including eleven laboratories from five different countries, was carried out to establish the HIV-1 RNA National Standard by calibration, in International Units (IU), against the WHO HIV-1 RNA IS. The HIV-1 RNA content for the candidate was quantitated by each laboratory in three independent assays and the results were collected and analyzed statistically. Overall, a high level of agreement among results was achieved from different laboratories. In addition, the stability study indicated that the candidate was stable for 24 months at -80 ± 5. °C. In conclusion, the candidate standard was established as the first National Standard for HIV-1 RNA for use in NAT assays in Taiwan. The standard is intended to be used for the quality control of HIV-1 NAT assays and as a quantitative reference material for HIV-1 NAT assays. ? 2013 Elsevier B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84878237417&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/380685 |
DOI: | 10.1016/j.jviromet.2013.04.002 | SDG/關鍵字: | article; bioassay; clinical laboratory; clinical study; controlled study; genotype; health care quality; human; Human immunodeficiency virus 1; nonhuman; nucleic acid amplification; priority journal; quality control; quantitative analysis; reference value; standard; Taiwan; HIV Infections; HIV-1; Humans; International Cooperation; Nucleic Acid Amplification Techniques; Reference Standards; RNA, Viral; Taiwan; Viral Load |
顯示於: | 生物科技研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。