|Title:||Development and characterization of tomato SSR markers from genomic sequences of anchored BAC clones on chromosome 6||Authors:||Subramaniam Geethanjali
Davidson V. Pastrana
|Keywords:||bacteria artificial chromosome;genetic diversity;linkage map;simple sequence repeats;Solanum lycopersicum||Issue Date:||2010||Journal Volume:||173||Start page/Pages:||85-97||Source:||Euphytica||Abstract:||
Simple sequence repeat motifs are abundant in plant genomes and are commonly used molecular markers in plant breeding. In tomato, currently available genetic maps possess a limited number of simple sequence repeat (SSR) markers that are not evenly distributed in the genome. This situation warrants the need for more SSRs in genomic regions lacking adequate markers. The objective of the study was to develop SSR markers pertaining to
chromosome 6 from bacterial artificial chromosome (BAC) sequences available at Solanaceae Genomics Network. A total of 54 SSR primer pairs from 17
BAC clones on chromosome 6 were designed and validated. Polymorphism of these loci was evaluated in a panel of 16 genotypes comprising of Solanum lycopersicum and its wild relatives. Genetic diversity analysis based on these markers could distinguish genotypes at species level. Twenty-one SSR markers
derived from 13 BAC clones were polymorphic between two closely related tomato accessions, West Virginia 700 and Hawaii 7996 and were mapped
using a recombinant inbred line population derived from a cross between these two accessions. The markers were distributed throughout the chromosome
spanning a total length of 117.6 cM following the order of the original BAC clones. A major QTL associated with resistance to bacterial wilt was
mapped on chromosome 6 at similar location of the reported Bwr-6 locus. These chromosome 6-specific SSR markers developed in this study are useful tools for cultivar identification, genetic diversity analysis and genetic mapping in tomato.
|Appears in Collections:||農藝學系|
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