Lectin-functionalized mesoporous silica nanoparticles for endoscopic detection of premalignant colonic lesions
Journal
Nanomedicine: Nanotechnology, Biology, and Medicine
Journal Volume
13
Journal Issue
6
Pages
1941-1952
Date Issued
2017
Author(s)
Chen N.-T.
Souris J.S.
Cheng S.-H.
Chu C.-H.
Wang Y.-C.
Konda V.
Dougherty U.
Bissonnette M.
Chen C.-T.
Lo L.-W.
Abstract
Colorectal cancer (CRC) is one of the leading causes of cancer-deaths worldwide. Methods for the early in situ detection of colorectal adenomatous polyps and their precursors – prior to their malignancy transformation into CRC – are urgently needed. Unfortunately at present, the primary diagnostic method, colonoscopy, can only detect polyps and carcinomas by shape/morphology; with sessile polyps more likely to go unnoticed than polypoid lesions. Here we describe our development of polyp-targeting, fluorescently-labeled mesoporous silica nanoparticles (MSNs) that serve as targeted endoscopic contrast agents for the early detection of colorectal polyps and cancer. In vitro cell studies, ex vivo histopathological analysis, and in vivo colonoscopy and endoscopy of murine colorectal cancer models, demonstrate significant binding specificity of our nanoconstructs to pathological lesions via targeting aberrant α-L-fucose expression. Our findings strongly suggest that lectin-functionalized fluorescent MSNs could serve as a promising endoscopic contrast agent for in situ diagnostic imaging of premalignant colonic lesions. ? 2017 Elsevier Inc.
Subjects
Colorectal tumors; Endoscopic; Mesoporous silica nanoparticle; Ulex europaeus Agglutinin 1 (UEA1)
SDGs
Other Subjects
Diseases; Endoscopy; Proteins; Silica nanoparticles; Binding specificities; Colorectal cancers (CRC); Colorectal tumors; Endoscopic; Histopathological analysis; Mesoporous silica nanoparticles; Pathological lesions; Ulex europaeus Agglutinin 1 (UEA1); Mesoporous materials; fluorescent dye; fucose; lectin; mesoporous silica nanoparticle; lectin; nanoparticle; silicon dioxide; animal experiment; animal model; animal tissue; Article; Caco-2 cell line; colonoscopy; colorectal cancer; colorectal polyp; controlled study; early cancer diagnosis; endoscopy; ex vivo study; HCT 116 cell line; histopathology; human; human cell; human tissue; in vivo study; male; mouse; nonhuman; precancer; A J mouse; animal; chemically induced; chemistry; colon; colon polyp; colorectal tumor; pathology; precancer; procedures; tumor cell culture; Animals; Colon; Colonic Polyps; Colonoscopy; Colorectal Neoplasms; Endoscopy; Fluorescent Dyes; Humans; Lectins; Male; Mice; Mice, Inbred A; Nanoparticles; Precancerous Conditions; Silicon Dioxide; Tumor Cells, Cultured
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
Lectin-functionalized mesoporous silica nanoparticles for endoscopic detection of premalignant colonic lesions.pdf
Size
2.08 MB
Format
Adobe PDF
Checksum
(MD5):5b5deec1c0dc026b9f89f66e21a997d3