Rapid SERS monitoring of lipid-peroxidation-derived protein modifications in cells using photonic crystal fiber sensor
Journal
Journal of Biophotonics
Journal Volume
9
Pages
32-37
Date Issued
2016
Author(s)
Gong, T.
Zhang, N.
Goh, D.
Ying, C.
Auguste, J.-L.
Shum, P.P.
Wei, L.
Humbert, G.
Yong, K.-T.
Olivo, M.
Abstract
We proposed a side channel photonic crystal fiber (SC-PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC-PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference-free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells.
SDGs
Type
letter