Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Engineering / 工學院
  3. Chemical Engineering / 化學工程學系
  4. Effects of glycation on human £^D-crystallin proteins by different glycation-inducing agents
 
  • Details

Effects of glycation on human £^D-crystallin proteins by different glycation-inducing agents

Journal
International Journal of Biological Macromolecules
Journal Volume
118
Pages
442-451
Date Issued
2018
Author(s)
Li C.-T.
How S.-C.
Chen M.-E.
Lo C.-H.
Chun M.-C.
Chang C.-K.
Chen W.-A.
Wu J.W.
Wang S.S.-S.  
DOI
10.1016/j.ijbiomac.2018.06.108
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/406708
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049078571&doi=10.1016%2fj.ijbiomac.2018.06.108&partnerID=40&md5=469d99e24c7c12546c1e03ea6e3a03c3
Abstract
Human £^D-crystallin (H£^D-crystallin), a major protein component of the human eye lens, is associated with the development of juvenile- and mature-onset cataracts. Evidence suggests that nonenzymatic protein glycation plays an important role in the aetiology of cataract and diabetic sequelae. This research compared the effects of various glycation modifiers on H£^D-crystallin aggregation, by treating samples of H£^D-crystallin with ribose, galactose, or methylglyoxal using several biophysical techniques. To measure advanced glycation end products, an N£`-(carboxyethyl)lysine enzyme-linked immunosorbent assay was performed on the glycating agent-treated H£^D-crystallin samples. Fructosamine production detection was performed for both ribose-treated and galactose-treated samples. Methylglyoxal-treated samples had the highest level of aggregation and the greatest extent of unfolding, and upon incubation for a minimum of 12 days, exhibited a marked enhancement in the amount of N£`-(carboxyethyl)lysine. The molecular profiles and morphological features of the glycated samples were highly correlated to the type of glycation agent used. These findings highlight a close connection between the type of glycation modifier and the various aggregation species that form. Thus, these results may facilitate deciphering of the molecular mechanism of diabetic cataractogenesis. ? 2018
SDGs

[SDGs]SDG3

Other Subjects
advanced glycation end product receptor affecting agent; fructosamine; galactose; gamma crystallin; gamma D crystallin; methylglyoxal; n epsilon(carboxyethyl)lysine; ribose; unclassified drug; advanced glycation end product; CRYGD protein, human; galactose; gamma crystallin; lysine; N(6)-carboxyethyllysine; ribose; absorption spectroscopy; Article; circular dichroism; controlled study; enzyme linked immunosorbent assay; human; hydrophobicity; incubation time; limit of quantitation; molecular weight; polyacrylamide gel electrophoresis; protein aggregation; protein expression; protein function; protein glycosylation; protein secondary structure; protein unfolding; spectrofluorometry; transmission electron microscopy; ultraviolet spectrophotometry; analogs and derivatives; biosynthesis; cataract; chemistry; complication; diabetic complication; drug effect; genetics; glycosylation; lens; metabolism; pathology; protein denaturation; proteinosis; Cataract; Diabetes Complications; Fructosamine; Galactose; gamma-Crystallins; Glycation End Products, Advanced; Glycosylation; Humans; Lens, Crystalline; Lysine; Protein Aggregation, Pathological; Protein Denaturation; Pyruvaldehyde; Ribose
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science