|Title:||Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions||Authors:||How S.-C.
|Issue Date:||2016||Journal Volume:||7||Journal Issue:||12||Start page/Pages:||4898-4907||Source:||Food and Function||Abstract:||
More than thirty human proteins and/or peptides can fold incorrectly to form amyloid deposits associated with several protein aggregation diseases. No cure is currently available for treating these diseases. This work is aimed at examining the inhibitory potency of fast green FCF, a biocompatible dye, toward the fibrillogenesis/aggregation of lysozyme. As verified by ThT binding assay along with transmission electron microscopy, fast green FCF was observed to suppress the generation of lysozyme fibrils in a concentration-dependent manner. We next used circular dichroism absorption spectroscopy, ANS fluorescence spectroscopy, and SDS-PAGE to characterize the structural alterations in lysozyme samples upon the addition of fast green FCF. Furthermore, experiments with the addition of fast green FCF at different time points of incubation showed that fast green FCF also exhibited disaggregating activity against the preformed/existing lysozyme fibrils. We believe that the results from this study suggest a potential therapeutic role of biocompatible molecules in treating or preventing protein aggregation diseases. ? 2016 The Royal Society of Chemistry.
Absorption spectroscopy; Biocompatibility; Circular dichroism spectroscopy; Dichroism; Enzymes; Fluorescence spectroscopy; Glycoproteins; High resolution transmission electron microscopy; Transmission electron microscopy; Acidic conditions; Amyloid deposits; Circular dichroism absorption; Concentration-dependent manners; Fast Green FCF; Fibrillogenesis; Protein aggregation disease; Structural alterations; Proteins; amyloid; dye; egg white; Fast Green FCF; lysozyme; thiazole derivative; thioflavine; animal; chemistry; chicken; circular dichroism; pH; Amyloid; Animals; Chickens; Circular Dichroism; Egg White; Hydrogen-Ion Concentration; Lissamine Green Dyes; Muramidase; Thiazoles
|Appears in Collections:||化學工程學系|
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